A novel and conserved protein AHO-3 is required for thermotactic plasticity associated with feeding states in Caenorhabditis elegans.
A novel and conserved protein AHO-3 is required for thermotactic plasticity associated with feeding states in Caenorhabditis elegans.
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DOI：
10.1111/j.1365-2443.2012.01594.x
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Mori I
中科院分区：
文献类型：
--
作者：
Nishio N;Mohri-Shiomi A;Nishida Y;Hiramatsu N;Kodama-Namba E;Kimura KD;Kuhara A;Mori I
尽管在神经系统中表达的大部分分子从无脊椎动物到脊椎动物都是保守的，但这些分子的功能特性很少被表征。在这里，我们表明，高度保守的水解酶AHO-3作为一种新的调节饥饿诱导的趋热可塑性秀丽隐杆线虫。作为野生型动物，aho-3突变体在特定温度下与食物一起培养后，以线性热梯度迁移到培养温度。而野生型动物在饥饿条件下培养的梯度上表现出分散的分布，aho-3突变体表现出向高温迁移的趋势。这种异常行为被AHO-3的人类同源物的表达完全拯救，表明AHO-3的分子功能在线虫和人类之间高度保守。AHO-3的行为调控需要N-末端半胱氨酸簇，这确保了AHO-3在感觉末梢的适当亚细胞定位。双突变体分析表明，AHO-3与ODR-3（一种异源三聚体G蛋白α亚基）在相同的途径中起作用。我们的结果揭示了一种新的神经蛋白在C。elegans，确认其在行为调节中的保守作用。
Although a large proportion of molecules expressed in the nervous system are conserved from invertebrate to vertebrate, functional properties of such molecules are less characterized. Here, we show that highly conserved hydrolase AHO-3 acts as a novel regulator of starvation-induced thermotactic plasticity in Caenorhabditis elegans. As wild-type animals, aho-3 mutants migrated to the cultivation temperature on a linear thermal gradient after cultivation at a particular temperature with food. Whereas wild-type animals cultivated under food-deprived condition showed dispersed distribution on the gradient, aho-3 mutants exhibited tendency to migrate toward higher temperature. Such an abnormal behavior was completely rescued by the expression of human homologue of AHO-3, indicating that the molecular function of AHO-3 is highly conserved between nematode and human. The behavioral regulation by AHO-3 requires the N-terminal cysteine cluster, which ensures the proper subcellular localization of AHO-3 to sensory endings. Double-mutant analysis suggested that AHO-3 acts in the same pathway with ODR-3, a heterotrimeric G protein alpha subunit. Our results unveiled a novel neural protein in C. elegans, confirming its conserved role in behavioral regulation.
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影响因子：
16.2
作者：
Gomez, M;De Castro, E;Nef, P
通讯作者：
Nef, P
影响因子：
64.5
作者：
BARGMANN, CI;HARTWIEG, E;HORVITZ, HR
通讯作者：
HORVITZ, HR
DOI：
10.1073/pnas.0805004105
发表时间：
2008-08-05
影响因子：
11.1
作者：
Biron, David;Wasserman, Sara;Sengupta, Piali
通讯作者：
Sengupta, Piali
影响因子：
64.8
作者：
HART, AC;SIMS, S;KAPLAN, JM
通讯作者：
KAPLAN, JM
影响因子：
16.2
作者：
Fujiwara, M;Sengupta, P;McIntire, SL
通讯作者：
McIntire, SL