Chromosomal location and comparative genomics analysis of powdery mildew resistance gene Pm51 in a putative wheat-Thinopyrum ponticum introgression line.
Chromosomal location and comparative genomics analysis of powdery mildew resistance gene Pm51 in a putative wheat-Thinopyrum ponticum introgression line.
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DOI：
10.1371/journal.pone.0113455
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chang Z
中科院分区：
文献类型：
--
作者：
Zhan H;Li G;Zhang X;Li X;Guo H;Gong W;Jia J;Qiao L;Ren Y;Yang Z;Chang Z
白粉病 (PM) 是一种对小麦 (Triticum aestivum L.) 极具破坏性的病害。小麦-Thinopyrum ponticum基因渗入品系CH7086被证明具有可能源自Th的白粉病抗性。桥体。基因组原位杂交和外来基因渗入的分子表征未能识别外来染色质。为了研究抗性遗传学，CH7086 与易感基因型杂交。在受控条件下用中国 Bgt 种族 E09 测试的 F2 群体和 F2:3 品系的分离表明，CH7086 携带单一的白粉病抗性显性基因。鉴定了 14 个与该基因座连锁的 SSR 和 EST-PCR 标记。该位点与两个侧翼标记之间的遗传距离分别为 1.5 和 3.2 cM。根据‘中国春’无效体-四体系和缺失系的标记位置，抗性基因位于缺失区2BL-0.89-1.00。保守同源标记分析表明，该抗性基因侧翼的基因组区域与水稻4号染色体和5号染色体具有高度共线性。抗性特异性和等位性测试均表明CH7086中的抗性基因与之前报道的2BL上的白粉病抗性基因不同，该基因暂命名为PmCH86。对PmCH86与2BL-0.89-1.00区域的其他抗Bgt基因进行分子分析表明，PmCH86可能是一个新的PM抗性基因，因此将其命名为Pm51。紧密相连的侧翼标记可用于利用这种假定的小麦-Thinopyrum 易位系，将 Pm51 快速转移到小麦育种计划中。
Powdery mildew (PM) is a very destructive disease of wheat (Triticum aestivum L.). Wheat-Thinopyrum ponticum introgression line CH7086 was shown to possess powdery mildew resistance possibly originating from Th. ponticum. Genomic in situ hybridization and molecular characterization of the alien introgression failed to identify alien chromatin. To study the genetics of resistance, CH7086 was crossed with susceptible genotypes. Segregation in F2 populations and F2:3 lines tested with Chinese Bgt race E09 under controlled conditions indicated that CH7086 carries a single dominant gene for powdery mildew resistance. Fourteen SSR and EST-PCR markers linked with the locus were identified. The genetic distances between the locus and the two flanking markers were 1.5 and 3.2 cM, respectively. Based on the locations of the markers by nullisomic-tetrasomic and deletion lines of ‘Chinese Spring’, the resistance gene was located in deletion bin 2BL-0.89-1.00. Conserved orthologous marker analysis indicated that the genomic region flanking the resistance gene has a high level of collinearity to that of rice chromosome 4 and Brachypodium chromosome 5. Both resistance specificities and tests of allelism suggested the resistance gene in CH7086 was different from previously reported powdery mildew resistance genes on 2BL, and the gene was provisionally designated PmCH86. Molecular analysis of PmCH86 compared with other genes for resistance to Bgt in the 2BL-0.89-1.00 region suggested that PmCH86 may be a new PM resistance gene, and it was therefore designated as Pm51. The closely linked flanking markers could be useful in exploiting this putative wheat-Thinopyrum translocation line for rapid transfer of Pm51 to wheat breeding programs.
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