Fabrication and characterization of biomimetic collagen-apatite scaffolds with tunable structures for bone tissue engineering.
Fabrication and characterization of biomimetic collagen-apatite scaffolds with tunable structures for bone tissue engineering.
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DOI：
10.1016/j.actbio.2013.03.038
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发表时间：
2013-07
影响因子：
9.7
通讯作者：
Wei, Mei
中科院分区：
文献类型：
--
作者：
Xia, Zengmin;Yu, Xiaohua;Jiang, Xi;Brody, Harold D.;Rowe, David W.;Wei, Mei
本研究的目的是制备一种仿生胶原-磷灰石（Col-Ap）支架，用于改善骨修复和再生。将仿生自组装技术与可控冷冻铸造技术相结合，提出了一种自下而上的方法。在这项研究中，矿化胶原纤维的生成使用一个简单的一步共沉淀法，其中包括胶原自组装和原位磷灰石沉淀在含胶原的改性模拟体液（m-SBF）。对沉淀物进行可控冷冻铸造，形成具有各向同性等轴结构或单向片状结构的支架。这些支架由胶原纤维和结晶性差的骨样碳酸磷灰石纳米颗粒组成。通过简单地调节m-SBF中的胶原蛋白含量，可以将支架中的矿物质含量调整在0-54重量%的范围内。进一步研究了等轴晶和片状晶支架的形成机制，建立了等轴晶和片状晶的凝固制度。最后，在小鼠颅骨缺损模型中体内评价这种制备的支架的成骨能力。证实了支架良好地支持新骨形成。
The objective of the current study is to prepare a biomimetic collagen-apatite (Col-Ap) scaffold for improved bone repair and regeneration. A novel bottom-up approach has been developed, which combines a biomimetic self-assembly method with a controllable freeze casting technology. In this study, the mineralized collagen fibers were generated using a simple one-step co-precipitation method which involved collagen self-assembly and in situ apatite precipitation in a collagen-containing modified simulated body fluid (m-SBF). The precipitates were subjected to controllable freeze casting, forming scaffolds with either an isotropic equiaxed structure or a unidirectional lamellar structure. These scaffolds were comprised of collagen fibers and poorly crystalline bone-like carbonated apatite nanoparticles. The mineral content in the scaffold could be tailored in a range 0–54 wt% by simply adjusting the collagen content in the m-SBF. Further, the mechanisms of the formation of both the equiaxed and the lamellar scaffolds were investigated, and freezing regimes for equiaxed and lamellar solidification were established. Finally, bone forming capability of such prepared scaffolds was evaluated in vivo in a mouse calvarial defect model. It was confirmed that the scaffolds well support new bone formation.
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影响因子：
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