Voluntary exercise offers anxiolytic potential and amplifies galanin gene expression in the locus coeruleus of the rat.
Voluntary exercise offers anxiolytic potential and amplifies galanin gene expression in the locus coeruleus of the rat.
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DOI：
10.1016/j.bbr.2012.05.001
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发表时间：
2012-07-15
影响因子：
2.7
通讯作者：
Holmes, Philip V.
中科院分区：
文献类型：
--
作者：
Sciolino, Natale R.;Dishman, Rodney K.;Holmes, Philip V.
虽然运动可以改善人类的焦虑，但运动是否能缓解啮齿动物的焦虑仍存在争议。我们检验了压力影响运动对焦虑样行为和防御行为的影响的假设。为了探索运动的神经生物学机制，我们还研究了运动是否会改变应激相关肽甘丙肽的基因表达。将大鼠在存在或不存在转轮的情况下圈养21 d。这些大鼠的一个亚组（1）在试验前即刻未注射或接受单次高剂量β-咔啉FG 7142（苯二氮卓类受体部位的反向激动剂），或（2）在最后10天运动期间重复注射溶媒或FG 7142。在第22天，在高架十字迷宫、电击探针防御性掩埋和防御性撤回测试中测量焦虑样和防御行为。原位杂交检测蓝斑前甘丙肽原mRNA的表达。未注射的运动和久坐大鼠在所有测试中表现出相似的行为，而在测试组合之前立即注射FG 7142产生了与焦虑样反应一致的强烈回避和不动。然而，相对于重复注射溶剂或FG 7142的大鼠的久坐状态，运动在试验组合中产生了抗焦虑样和主动防御行为。相对于久坐不动的对照组，运动还增加了蓝斑的前甘丙肽原mRNA。这些数据表明，增强适应行为的出现后，慢性自愿运动的压力。我们的数据支持甘丙肽在车轮运行的有益后果中的作用。
Although exercise improves anxiety in humans, it is controversial whether exercise is anxiolytic in rodents. We tested the hypothesis that stress influences the effect of exercise on anxiety-like and defensive behaviors. To explore the neurobiological mechanisms of exercise, we also examined whether exercise alters gene expression for the stress-related peptide galanin. Rats were housed in the presence or absence of a running wheel for 21 d. A subset of these rats were (1) not injected or received a single high, dose of the β-carboline FG7142 (inverse agonist at the benzodiazepine receptor site) immediately prior to testing or (2) were injected repeatedly with vehicle or FG7142 during the last 10 d of exercise. On day 22, anxiety-like and defensive behaviors were measured in the elevated plus maze, shock probe defensive burying, and defensive withdrawal tests. Locus coeruleus prepro-galanin mRNA was measured by in situ hybridization. Exercise and sedentary rats that were not injected exhibited similar behavior in all tests, whereas FG7142 injected immediately prior to the test battery produced intense avoidance and immobility consistent with an anxiety-like response. However, exercise produced anxiolytic-like and active defensive behaviors in the test battery relative to the sedentary condition in rats injected repeatedly with vehicle or FG7142. Exercise also increased prepro-galanin mRNA in the locus coeruleus relative to sedentary controls. These data suggest that the emergence of enhanced adaptive behavior after chronic voluntary exercise is influenced by stress. Our data support a role for galanin in the beneficial consequences of wheel running.
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影响因子：
2.9
作者：
Burghardt, PR;Fulk, LJ;Wilson, MA
通讯作者：
Wilson, MA
影响因子：
4.7
作者：
Brocardo, Patricia S.;Boehme, Fanny;Christie, Brian R.
通讯作者：
Christie, Brian R.
影响因子：
3.2
作者：
Campeau S;Nyhuis TJ;Sasse SK;Kryskow EM;Herlihy L;Masini CV;Babb JA;Greenwood BN;Fleshner M;Day HE
通讯作者：
Day HE
影响因子：
3.8
作者：
Dishman, RK;Renner, KJ;Meyerhof, JL
通讯作者：
Meyerhof, JL
影响因子：
8.2
作者：
Blanchard, DC;Griebel, G;Blanchard, RJ
通讯作者：
Blanchard, RJ