Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo.
Thallium-201 Imaging in Intact Olfactory Sensory Neurons with Reduced Pre-Synaptic Inhibition In Vivo.
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DOI：
10.1007/s12035-020-02078-y
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发表时间：
2020-12
影响因子：
5.1
通讯作者：
Miwa T
中科院分区：
文献类型：
--
作者：
Shiga H;Wakabayashi H;Washiyama K;Noguchi T;Hiromasa T;Miyazono S;Kumai M;Ogawa K;Taki J;Kinuya S;Miwa T
在这项研究中，我们确定是否201铊（铊-201）为基础的嗅觉成像受到影响，如果嗅觉感觉神经元在体内接受减少的突触前抑制信号从嗅球多巴胺能中间神经元。在小鼠（每组6-7只动物）中，在左侧鼻施用鱼藤酮（一种线粒体呼吸链复合物I抑制剂，其减少多巴胺能中间神经元的数量而不损伤嗅球中的嗅感觉神经元）后3小时，评估铊-201向嗅球的迁移速率和嗅感觉神经元的动作电位的数量。与对照组小鼠相比，经鼻给予铊-201和鱼藤酮（10 μg鱼藤酮，p = 0.0012; 20 μg鱼藤酮，p = 0.0012）后，铊-201向嗅球的迁移率显著增加。与对照小鼠相比，20 μg鱼藤酮处理小鼠的鱼藤酮处理侧的嗅觉感觉神经元中的动作电位数量显著减少（p = 0.0029）。与对照组大鼠相比，在左侧鼻内给予铊-201和100 μg鱼藤酮后24 h，用SPECT-CT评估的铊-201向嗅球的迁移率显著增加（p = 0.008，每组5只大鼠）。我们的研究结果表明，铊-201迁移到嗅球增加完整的嗅觉感觉神经元，减少突触前抑制多巴胺能中间神经元在嗅球肾小球。本文的在线版本（10.1007/s12035-020-02078-y）包含补充材料，可供授权用户使用。
In this study, we determined whether the 201Tl (thallium-201)-based olfactory imaging is affected if olfactory sensory neurons received reduced pre-synaptic inhibition signals from dopaminergic interneurons in the olfactory bulb in vivo. The thallium-201 migration rate to the olfactory bulb and the number of action potentials of olfactory sensory neurons were assessed 3 h following left side nasal administration of rotenone, a mitochondrial respiratory chain complex I inhibitor that decreases the number of dopaminergic interneurons without damaging the olfactory sensory neurons in the olfactory bulb, in mice (6–7 animals per group). The migration rate of thallium-201 to the olfactory bulb was significantly increased following intranasal administration of thallium-201 and rotenone (10 μg rotenone, p = 0.0012; 20 μg rotenone, p = 0.0012), compared with that in control mice. The number of action potentials was significantly reduced in the olfactory sensory neurons in the rotenone treated side of 20 μg rotenone-treated mice, compared with that in control mice (p = 0.0029). The migration rate of thallium-201 to the olfactory bulb assessed with SPECT-CT was significantly increased in rats 24 h after the left intranasal administration of thallium-201 and 100 μg rotenone, compared with that in control rats (p = 0.008, 5 rats per group). Our results suggest that thallium-201 migration to the olfactory bulb is increased in intact olfactory sensory neurons with reduced pre-synaptic inhibition from dopaminergic interneurons in olfactory bulb glomeruli. The online version of this article (10.1007/s12035-020-02078-y) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
Shiga H;Taki J;Okuda K;Watanabe N;Tonami H;Nakagawa H;Kinuya S;Miwa T
通讯作者：
Miwa T
影响因子：
3.1
作者：
Kanayama, Y;Enomoto, S;Amano, R
通讯作者：
Amano, R
影响因子：
2.5
作者：
MORAN, DT;JAFEK, BW;ROWLEY, JC
通讯作者：
ROWLEY, JC
影响因子：
8.6
作者：
Huisman, E;Uylings, HBM;Hoogland, PV
通讯作者：
Hoogland, PV
影响因子：
3.3
作者：
Noguchi, Tomohiro;Miyazono, Sadaharu;Kashiwayanagi, Makoto
通讯作者：
Kashiwayanagi, Makoto