Microparticle profile and procoagulant activity of fresh-frozen plasma is affected by whole blood leukoreduction rather than 24-hour room temperature hold.
Microparticle profile and procoagulant activity of fresh-frozen plasma is affected by whole blood leukoreduction rather than 24-hour room temperature hold.
复制标题
DOI：
10.1111/trf.12602
复制
发表时间：
2014-08
期刊：
影响因子：
2.9
通讯作者：
Sparrow RL
中科院分区：
文献类型：
--
作者：
Chan KS;Sparrow RL
微粒(MPS)是一种含有磷脂的小泡，具有促凝血特性。MPS被认为对血浆的止血潜力有贡献。本研究探讨了WB保持时间和去白细胞(LD)对新鲜冰冻血浆(FFP)MP谱和止血潜力的影响。取健康供者的WB单位(n=12)，分成两对，每对在20~24℃下放置6或24小时。在指定的等待时间，这对人中的一个单位是去白细胞的，而另一个单位没有去白细胞。FFP按标准程序制备，等量冷冻。用绝对计数法和红细胞(CD235a)、血小板(CD41)和磷脂酰丝氨酸特异性标记的流式细胞仪测定MP含量。采用血栓弹力图(TEG)和凝血因子测定仪测定止血能力。与非LD FFP相比，LD-FFP的MPS数量显著减少，尤其是CD41+MPS和磷脂酰丝氨酸阳性MPS(p<；0.03)。与非LD-FFP相比，LD-FFP的血栓形成时间较慢(p=0.002)，凝血强度较低(p<；0.001)，FVIII、FxⅡ和纤维蛋白原水平较低(p<；0.01)。随着WB保持时间的延长，TEG曲线没有变化，尽管FVIII水平如预期的那样下降(p<；0.01)。平均而言，FFP单元符合质量要求。WB的LD降低了FFP的止血潜力，并伴有MPS和凝血因子的耗竭。根据TEG的测量，较长的WB停留时间对FFP的止血潜力没有显著影响。在所有研究的FFP工艺条件下，止血质量都保持在可接受的水平。
Microparticles (MPs) are small phospholipid-containing vesicles that have pro-coagulant properties. MPs are thought to contribute to the hemostatic potential of plasma. This study investigated the effects of WB-hold time and leukodepletion (LD) on the MP profile and hemostatic potential of fresh-frozen plasma (FFP). WB units (n=12) from healthy donors were divided into two pairs and each pair was held at 20–24°C for 6 or 24 hours. At the designated hold-time, one unit from the pair was leukodepleted while the other unit was not leukodepleted. FFP was prepared by standard procedures, aliquoted and frozen. The MP content was determined by flow cytometry using an absolute count assay and specific labels for red cells (CD235a), platelets (CD41) and phosphatidylserine. The hemostatic potential was determined by thrombelastography (TEG) and coagulation factor assays. Compared to non-LD FFP, LD-FFP had significantly lower numbers of MPs, particularly CD41+ MPs and phosphatidylserine-positive MPs (p<0.03). LD-FFP, compared to non-LD FFP, had a slower clot formation time (p=0.002), lower clot strength (p<0.001) and lower FVIII, FXII and fibrinogen levels (p<0.01). With longer WB hold-time, the TEG profile was unchanged, although FVIII levels were decreased as expected (p<0.01). On average FFP units met quality requirements. LD of WB resulted in lower hemostatic potential of FFP in conjunction with depletion of MPs and coagulation factors. Longer WB hold-time did not significantly affect the hemostatic potential of FFP as measured by TEG. Acceptable hemostatic quality was maintained for all FFP processing conditions studied.
登录
查看更多内容
影响因子：
1.5
作者：
Heiden, M;Salge, U;Seitz, R
通讯作者：
Seitz, R
影响因子：
1.1
作者：
Bochsen, Louise;Johansson, Par I.;Ostrowski, Sisse R.
通讯作者：
Ostrowski, Sisse R.
影响因子：
2.7
作者：
Lawrie, A. S.;Cardigan, R. A.;Mackie, I. J.
通讯作者：
Mackie, I. J.
影响因子：
2.7
作者：
Chudziak, D.;Sireis, W.;Boenig, H.
通讯作者：
Boenig, H.
影响因子：
2.9
作者：
Glenister, Kristen M.;Sparrow, Rosemary L.
通讯作者：
Sparrow, Rosemary L.