Evaporation of serum after long-term biobank storage: A chemical analysis of maternal serum from a large Danish pregnancy screening registry.
Evaporation of serum after long-term biobank storage: A chemical analysis of maternal serum from a large Danish pregnancy screening registry.
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DOI：
10.1371/journal.pone.0293527
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
在探索环境妊娠暴露和后代健康的流行病学研究中，依靠冷冻储存的生物标本是首选。储存时间可能会增加分析前的变异性，从而可能增加测量不确定性。我们研究了在长期生物样本库储存后母体血清的蒸发，使用公认的人血清稳定性和相对较窄的正常生物参考范围的离子（钠，Na+;氯化物，Cl-）。一项来自丹麦大型妊娠筛查登记处的275例生物库中期妊娠母体血清的化学分析研究。血清样本收集于1985-1995年之间，并储存在-20 ° C下。使用Roche Cobas 6000分析仪通过间接电位法定量离子浓度，并根据储存时间和孕中期正常生物范围进行比较。还将离子浓度与通过基线Na+和Cl-血清浓度评估的正常生物学变化进行了比较，该基线Na+和Cl-血清浓度来自使用相同仪器在冷冻前测量的24，199名非妊娠女性的单独队列。生物库血清中的总体平均离子浓度为147.5 mmol/L（Na+）和109.7 mmol/L（Cl-）。根据储存时间，未观察到显著的线性储存效应。离子浓度在采样年份，特别是特定的采样年份，一直很高，并有相对较大的比例在孕中期超出各自的正常范围：38.9%的Na+和43.6%的Cl-。在用作质控品的基线血清中，浓度的一些变化也很明显。离子浓度升高表明蒸发，但在本研究中（27-37年）独立于储存时间。任何蒸发都可能发生在冷冻储存之前或前27年。其他分析前因素（如低血清体积）可能影响浓度，特别是考虑到年内变异性较高。总体而言，我们认为生物库血清样本具有内部可比性，可用于流行病学研究。
Relying on freezer stored biospecimens is preferred in epidemiolocal studies exploring environmental pregnancy exposures and later offspring health. Storage duration may increase the pre-analytical variability, potentially adding measurement uncertainty. We investigated evaporation of maternal serum after long-term biobank storage using ions (sodium, Na+; chloride, Cl-) recognized for stability and relatively narrow normal biological reference ranges in human serum. A chemical analysis study of 275 biobanked second trimester maternal serum from a large Danish pregnancy screening registry. Serum samples were collected between 1985–1995 and stored at -20°C. Ion concentrations were quantified with indirect potentiometry using a Roche Cobas 6000 analyzer and compared according to storage time and normal biological ranges in second trimester. Ion concentrations were also compared with normal biological variation assessed by baseline Na+ and Cl- serum concentrations from a separate cohort of 24,199 non-pregnant women measured before freezing with the same instrument. The overall mean ion concentrations in biobanked serum were 147.5 mmol/L for Na+ and 109.7 for Cl-. No marked linear storage effects were observed according to storage time. Ion concentrations were consistently high across sampling years, especially for specific sampling years, and a relatively large proportion were outside respective normal ranges in second trimester: 38.9% for Na+ and 43.6% for Cl-. Some variation in concentrations was also evident in baseline serum used as quality controls. Elevated ion concentrations suggest evaporation, but independent of storage duration in the present study (27–37 years). Any evaporation may have occurred prior to freezer storage or during the first 27 years. Other pre-analytical factors such as low serum volume have likely influenced the concentrations, particularly given the high within year variability. Overall, we consider the biobanked serum samples internally comparable to enable their use in epidemiological studies.
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影响因子：
3.7
作者：
Bach CC;Henriksen TB;Bossi R;Bech BH;Fuglsang J;Olsen J;Nohr EA
通讯作者：
Nohr EA
影响因子：
3.1
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影响因子：
13.6
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通讯作者：
Hoppin, Jane A.
影响因子：
8.1
作者：
Bone JN;Pickerill K;Woo Kinshella ML;Vidler M;Craik R;Poston L;Stones W;Sevene E;Temmerman M;Koech Etyang A;Roca A;Russell D;Tribe RM;von Dadelszen P;Magee LA;PRECISE Network
通讯作者：
PRECISE Network
影响因子：
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作者：
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