Global feather orientations changed by electric current.
Global feather orientations changed by electric current.
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DOI：
10.1016/j.isci.2021.102671
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发表时间：
2021-06-25
期刊：
影响因子：
5.8
通讯作者：
Chuong CM
中科院分区：
文献类型：
--
作者：
Jiang TX;Li A;Lin CM;Chiu C;Cho JH;Reid B;Zhao M;Chow RH;Widelitz RB;Chuong CM
在鸡皮的发育过程中，每个羽毛芽都有自己的极性，但一群芽的组织具有集体的全局方向。我们使用胚胎背部皮肤，芽对齐平行于喙尾体轴，探讨是否外源电场影响羽毛极性。有趣的是，短暂的外源电流暴露之前，可见芽形成后来改变芽的方向。施加垂直于身体喙尾轴的电脉冲，使芽的生长重新排列成集体漩涡，类似于指向阳极的电场。扰动芽显示正常的分子表达和形态发生，除了它们的方向改变。上皮-间充质重组表明外源电场的作用是通过上皮介导的。小分子通道抑制剂筛选显示Ca 2+通道和PI 3激酶参与控制羽芽极性。这项工作揭示了生物电在器官发育和再生中的重要性，并为实验提供了外植体培养平台。3脉冲电刺激共同改变了羽毛的方向电场拓扑结构重塑了上皮中的极性线索扰动暗示了Ca 2+通道的参与，而不是平面细胞极性分子表征表明羽毛芽是正常的生物科学;细胞生物学;发育生物学
During chicken skin development, each feather bud exhibits its own polarity, but a population of buds organizes with a collective global orientation. We used embryonic dorsal skin, with buds aligned parallel to the rostral-caudal body axis, to explore whether exogenous electric fields affect feather polarity. Interestingly, brief exogenous current exposure prior to visible bud formation later altered bud orientations. Applying electric pulses perpendicular to the body rostral-caudal axis realigned bud growth in a collective swirl, resembling an electric field pointing toward the anode. Perturbed buds show normal molecular expression and morphogenesis except for their altered orientation. Epithelial-mesenchymal recombination demonstrates the effects of exogenous electric fields are mediated through the epithelium. Small-molecule channel inhibitor screens show Ca2+ channels and PI3 Kinase are involved in controlling feather bud polarity. This work reveals the importance of bioelectricity in organ development and regeneration and provides an explant culture platform for experimentation. Feather orientation is collectively altered by 3-pulsed electrical stimulations The electric field topology reshapes polarity cues in the epithelium Perturbation implicates involvement of Ca2+ channels, not planar cell polarity Molecular characterization shows feather buds are otherwise normal Biological sciences; Cell biology; Developmental biology
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DOI：
10.1016/j.bbamcr.2016.10.010
发表时间：
2017-06
期刊：
Biochimica et biophysica acta. Molecular cell research
影响因子：
--
作者：
Chan JD;Zhang D;Liu X;Zarowiecki M;Berriman M;Marchant JS
通讯作者：
Marchant JS
影响因子：
3.3
作者：
Golberg, Alexander;Villiger, Martin;Yarmush, Martin L.
通讯作者：
Yarmush, Martin L.
影响因子：
9.2
作者：
Allen, Greg M.;Mogilner, Alex;Theriot, Julie A.
通讯作者：
Theriot, Julie A.
DOI：
10.1073/pnas.0402802101
发表时间：
2004-06-22
影响因子：
11.1
作者：
Guo, N;Hawkins, C;Nathans, J
通讯作者：
Nathans, J
影响因子：
6.5
作者：
Chuong, CM;Widelitz, RB;Jiang, TX
通讯作者：
Jiang, TX