Generation of neurospheres from human adipose-derived stem cells.
Generation of neurospheres from human adipose-derived stem cells.
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从人类脂肪干细胞生成神经球
DOI：
10.1155/2015/743714
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发表时间：
2015
影响因子：
--
通讯作者：
Tang Z
中科院分区：
文献类型：
--
作者：
Yang E;Liu N;Tang Y;Hu Y;Zhang P;Pan C;Dong S;Zhang Y;Tang Z
神经干细胞(NSCs)移植治疗神经退行性疾病前景看好，但NSCs的临床应用受到侵入性获取和伦理问题的限制。脂肪来源的干细胞(ADSCs)是一种多能干细胞的来源，可以自我更新并分化为各种细胞；本研究旨在通过将ADSCs培养在含有B27、碱性成纤维细胞生长因子(BFGF)和表皮生长因子(EGF)的无血清神经基础培养瓶中，将ADSCs培养成神经球；ADSCs来源的神经球在生长因子停用后被终末分化。用定量逆转录聚合酶链式反应(qRT-PCR)、免疫印迹和免疫细胞化学方法检测ADSCs和终末分化神经球中Nestin、NeuN、MAP2和GFAP的表达，细胞周期分析、免疫染色和流式细胞仪检测细胞增殖情况。这些数据有力地支持了人ADSCs可以在无涂层培养瓶中高效分化为神经球的结论，该培养瓶具有与胚胎和成人脑组织来源的NSCs相似的分子标记模式和增殖能力。因此，人ADSCs可能是治疗神经退行性疾病的理想的干细胞替代来源。
Transplantation of neural stem cells (NSCs) to treat neurodegenerative disease shows promise; however, the clinical application of NSCs is limited by the invasive procurement and ethical concerns. Adipose-derived stem cells (ADSCs) are a source of multipotent stem cells that can self-renew and differentiate into various kinds of cells; this study intends to generate neurospheres from human ADSCs by culturing ADSCs on uncoated culture flasks in serum-free neurobasal medium supplemented with B27, basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF); the ADSCs-derived neurospheres were terminally differentiated after growth factor withdrawal. Expression of Nestin, NeuN, MAP2, and GFAP in ADSCs and terminally differentiated neurospheres was shown by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), western blotting, and immunocytochemistry; cell proliferation in neurospheres was evaluated by cell cycle analyses, immunostaining, and flow cytometry. These data strongly support the conclusion that human ADSCs can successfully differentiate into neurospheres efficiently on uncoated culture flasks, which present similar molecular marker pattern and proliferative ability with NSCs derived from embryonic and adult brain tissues. Therefore, human ADSCs may be an ideal alternative source of stem cells for the treatment of neurodegenerative diseases.
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影响因子：
0.9
作者：
Nagase, Takashi;Matsumoto, Daisuke;Machida, Masafumi
通讯作者：
Machida, Masafumi
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
5.3
作者：
Carpenter, MK;Cui, X;Wahlberg, LU
通讯作者：
Wahlberg, LU
影响因子：
2.6
作者：
Ahmadi, Nafiseh;Razavi, Shahnaz;Oryan, Shahrbanoo
通讯作者：
Oryan, Shahrbanoo
影响因子：
3.6
作者：
Ashjian, PH;Elbarbary, AS;Hedrick, MH
通讯作者：
Hedrick, MH