AoSte12 Is Required for Mycelial Development, Conidiation, Trap Morphogenesis, and Secondary Metabolism by Regulating Hyphal Fusion in Nematode-Trapping Fungus Arthrobotrys oligospora.
AoSte12 Is Required for Mycelial Development, Conidiation, Trap Morphogenesis, and Secondary Metabolism by Regulating Hyphal Fusion in Nematode-Trapping Fungus Arthrobotrys oligospora.
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DOI：
10.1128/spectrum.03957-22
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发表时间：
2023-02-14
影响因子：
3.7
通讯作者：
Yang, Jinkui
中科院分区：
文献类型：
--
作者：
Bai, Na;Xie, Meihua;Liu, Qianqian;Wang, Wenjie;Liu, Yankun;Yang, Jinkui
关键词：
诱捕线虫（NT）真菌是一类独特的肉食性微生物，它们可以通过制造巧妙的诱捕装置（陷阱）来捕获和消化线虫。寡孢节肢真菌（Arthrobotrys oligospora）是NT真菌的代表，它可以形成粘附的三维网络来捕食线虫。菌丝融合是小孢子草形成陷阱的必要条件。在这里，我们通过基因破坏、DNA亲和纯化测序（DAP-Seq）和多组学方法表征了A. oligospora的同源Ste12蛋白（AoSte12）。Aoste12基因的破坏导致菌丝融合增加，导致菌丝生长、分生、陷阱形态和抗逆性缺陷，细胞核数量和脂滴积累减少。此外，转录组和DAP-Seq分析显示，AoSte12参与了与生长、细胞融合、三羧酸循环、囊泡、肌动蛋白丝和脂质代谢相关的细胞过程。此外，结合代谢组与转录组和DAP-Seq分析表明，AoSte12参与了丝裂原活化蛋白激酶信号通路、脂质代谢和次生代谢产物。酵母双杂交实验表明，AoSte12可以与多种蛋白相互作用，如mak2同源蛋白Fus3、液泡分选蛋白Pep3和udp -糖基转移酶。研究结果表明，AoSte12在菌丝融合过程中起着不可缺少的作用，从而调控孢子形成和陷阱形态发生。这些结果为NT真菌菌丝融合与陷阱形成之间的联系提供了深入的见解。诱捕线虫真菌是线虫的重要天敌，可以通过制造诱捕器捕获其猎物。菌丝吻合和融合对丝状真菌菌丝生长和菌落形态发育具有重要意义，对NT真菌的陷阱形态形成也至关重要。当感知到线虫的存在时，少孢子节肢虫可以形成复杂的三维网络（陷阱）。本研究表明，AoSte12在小孢子草菌丝融合中起着不可缺少的作用，它调节着小孢子草菌丝的生长、分生、陷阱形态发生、抗逆性、核数和脂滴积累。此外，DNA亲和纯化测序、转录组和代谢组分析进一步表明，AoSte12参与了丝裂原活化蛋白激酶途径、脂质代谢和次级代谢。总之，这些发现拓展了AoSte12在NT真菌a . oligospora中的重要作用，为阐明病原菌陷阱发育和生活方式转变的调控机制提供了广泛的基础。
Nematode-trapping (NT) fungi are a unique group of carnivorous microorganisms that can capture and digest nematodes by producing ingenious trapping devices (traps). Arthrobotrys oligospora, a representative NT fungus, can develop adhesive three-dimensional networks for nematode predation. Hyphal fusion is indispensable for the trap formation of A. oligospora. Here, we characterized an orthologous Ste12 protein (AoSte12) in A. oligospora via gene disruption, DNA affinity purification sequencing (DAP-Seq), and multi-omics approaches. The disruption of the Aoste12 gene caused an increase in hyphal fusion and resulted in defects in mycelial growth, conidiation, trap morphology, and stress resistance, as well as reducing the number of nuclei and lipid droplet accumulation. Moreover, transcriptome and DAP-Seq analysis revealed that AoSte12 was involved in cellular processes associated with growth, cell fusion, the tricarboxylic acid cycle, vesicles, actin filaments, and lipid metabolism. In addition, combining metabolome with transcriptome and DAP-Seq analysis indicated that AoSte12 was involved in the mitogen-activated protein kinase signaling pathway, lipid metabolism, and secondary metabolites. A yeast two-hybrid assay revealed that AoSte12 can interact with diverse proteins, such as the MAK-2 orthologue protein Fus3, the vacuolar sorting protein Pep3, and UDP-glycosyltransferase. Our results suggest that AoSte12 plays an indispensable role in hyphal fusion and thus regulates sporulation and trap morphogenesis. These results provide deep insights into the connection between hyphal fusion and trap formation in NT fungi. IMPORTANCE Nematode-trapping (NT) fungi are an important natural enemy of nematodes and can capture their prey by producing traps. Hyphal anastomosis and fusion are important for mycelial growth and the colony morphological development of filamentous fungi and are also crucial for the trap morphogenesis of NT fungi. Arthrobotrys oligospora can form complex three-dimensional networks (traps) when sensing the presence of nematodes. This study revealed that AoSte12 is indispensable for hyphal fusion and that it regulates mycelial growth, conidiation, trap morphogenesis, stress resistance, the number of nuclei, and lipid droplet accumulation in A. oligospora. In addition, DNA affinity purification sequencing, transcriptome, and metabolome analyses further revealed that AoSte12 is involved in the mitogen-activated protein kinase pathway, lipid metabolism, and secondary metabolism. Overall, these findings expand the important role of AoSte12 in NT fungus A. oligospora and provide a broad foundation for elucidating the regulatory mechanism of trap development and the lifestyle transitions of pathogenic fungi.
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影响因子：
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作者：
Blackwell, Ernest;Kim, Hye-Jin N.;Stone, David E.
通讯作者：
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影响因子：
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DOI：
10.3390/jof8030260
发表时间：
2022-03-03
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Journal of fungi (Basel, Switzerland)
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影响因子：
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通讯作者：
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DOI：
10.3390/jof6040191
发表时间：
2020-09-27
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
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作者：
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通讯作者：
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