Recent trends in SELEX technique and its application to food safety monitoring.
Recent trends in SELEX technique and its application to food safety monitoring.
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DOI：
10.1007/s00604-013-1156-7
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发表时间：
2014-04
影响因子：
5.7
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Wu, Jingjing;Zhu, Yingyue;Xue, Feng;Mei, Zhanlong;Yao, Li;Wang, Xin;Zheng, Lei;Liu, Jian;Liu, Guodong;Peng, Chifang;Chen, Wei
被称为“指数富集配体系统进化”（SELEX）的方法于1990年引入，并且从那时起已经成为鉴定和筛选适体的重要工具。这样的核酸可以以高选择性和亲和力识别并结合其相应的靶标（分析物），因此适体已经成为传统抗体的有吸引力的替代品，这不仅仅是因为它们更稳定。与此同时，它们在包括食品质量和安全监测在内的不同领域得到了广泛的应用。本文首先介绍了核酸适体的筛选过程和SELEX技术的发展，然后介绍了核酸适体在食品安全监测中的应用（包括吸光光度法、电化学法、荧光法等），最后给出了结论和展望。SELEX方法具有体外、高通量、操作简便等优点。本文共引用文献86篇。
The method referred to as “systemic evolution of ligands by exponential enrichment” (SELEX) was introduced in 1990 and ever since has become an important tool for the identification and screening of aptamers. Such nucleic acids can recognize and bind to their corresponding targets (analytes) with high selectivity and affinity, and aptamers therefore have become attractive alternatives to traditional antibodies not the least because they are much more stable. Meanwhile, they have found numerous applications in different fields including food quality and safety monitoring. This review first gives an introduction into the selection process and to the evolution of SELEX, then covers applications of aptamers in the surveillance of food safety (with subsections on absorptiometric, electrochemical, fluorescent and other methods), and then gives conclusions and perspectives. The SELEX method excels by its features of in vitro, high throughput and ease of operation. This review contains 86 references.
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