Oral Microbiome: Streptococcus mutans/Caries Concordant-Discordant Children.
Oral Microbiome: Streptococcus mutans/Caries Concordant-Discordant Children.
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DOI：
10.3389/fmicb.2022.782825
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发表时间：
2022
影响因子：
5.2
通讯作者：
Tran NC
中科院分区：
文献类型：
--
作者：
Dinis M;Agnello M;Cen L;Shokeen B;He X;Shi W;Wong DTW;Lux R;Tran NC
龋齿仍然是儿童最常见的慢性疾病，其病因尚不完全清楚。尽管变形链球菌是龋齿发生和进展的重要因素，但它的存在并不总是与该疾病相关。龋齿不一致群体的存在，其中变形链球菌计数与龋齿经历不相关，提出了一个具有挑战性的问题。本研究探讨了变形链球菌和其他微生物水平与龋齿一致和不一致种群的龋齿相关生态学的可能相关性。本研究对总共 47 名儿童进行了分析，并根据唾液中的变形链球菌水平（HS/LS：高/低变形链球菌）和龋齿经历分为四个临床组。通过基于培养的选择性平板接种来测定变形链球菌水平。通过 16S rRNA 基因测序和下游生物信息学分析研究龋齿一致和不一致群体的唾液微生物组。唾液微生物群落根据变形链球菌水平显着聚集，且与其龋齿经历无关。除了变形链球菌水平外，HS 组和 LS 组之间其他物种的丰度也存在显着差异。有趣的是，与疾病相关的物种，如迪斯帕韦荣球菌、链球菌属和普雷沃菌属。在 HS 组中显着增加，并且可能与变形链球菌结合导致龋齿进展。此外，与健康相关的物种在 LS 组中表现出较高的丰度，例如 Veillonella rogosae、Haemophilus sp. 和 Alloprevotella spp。但它们对龋齿过程的可能贡献仍有待阐明。这项研究提供了证据表明变形链球菌可能在塑造唾液微生物群落中发挥作用。我们的结果强调，未来的龋齿研究应考虑将其他物种与变形链球菌结合作为健康/疾病微生物标志物，以改善对龋齿不一致人群的诊断和龋齿管理。
Dental caries remains the most common chronic disease in children, and the respective etiology is not fully understood. Though Streptococcus mutans is an important factor in the initiation and progression of caries, its presence is not always associated with the disease. The existence of caries discordant populations, in which S. mutans counts do not correlate with caries experience, poses a challenging problem. This study explored the possible correlation of S. mutans and other microorganism levels on caries-associated ecology of caries-concordant and discordant populations. A total of forty-seven children were analyzed in this study and stratified into four clinical groups based on their S. mutans levels in saliva (HS/LS: High/low S. mutans) and caries experience. Streptococcus mutans levels were determined by culture-based selective plating. The salivary microbiome of caries concordant and discordant populations was investigated by 16S rRNA gene sequencing and downstream bioinformatics analysis. The salivary microbial communities significantly clustered based on S. mutans levels and independent of their caries experience. In addition to S. mutans levels, significant differences in the abundance of other species were observed between HS and LS groups. Interestingly, disease-associated species such as Veillonella dispar, Streptococcus spp., and Prevotella spp. were significantly increased in HS groups and may contribute, in combination with S. mutans, to the caries progression. Furthermore, health-associated species exhibited higher abundance in the LS groups, such as Veillonella rogosae, Haemophilus sp., and Alloprevotella spp. but their possible contribution to the caries process remains to be elucidated. This study provides evidence that S. mutans may play a role in shaping the salivary microbial community. Our results highlight that future caries research should consider additional species as health/disease microbial markers in conjunction with S. mutans to improve diagnosis and caries management of the caries-discordant population.
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影响因子：
2.9
作者：
Valdez RMA;Duque C;Caiaffa KS;Dos Santos VR;Loesch MLA;Colombo NH;Arthur RA;Negrini TC;Boriollo MFG;Delbem ACB
通讯作者：
Delbem ACB
影响因子：
5.7
作者：
Do T;Sheehy EC;Mulli T;Hughes F;Beighton D
通讯作者：
Beighton D
影响因子：
2.3
作者：
Henne, Karsten;Rheinberg, Anke;Conrads, Georg
通讯作者：
Conrads, Georg
影响因子：
7.6
作者：
Brailsford, SR;Tregaskis, RB;Beighton, D
通讯作者：
Beighton, D
无龋齿和受龋齿影响的儿童的唾液微生物组多样性。
影响因子：
5.6
作者：
Jiang S;Gao X;Jin L;Lo EC
通讯作者：
Lo EC