Molecular Identification of Multidrug-Resistant Campylobacter Species From Diarrheal Patients and Poultry Meat in Shanghai, China.
Molecular Identification of Multidrug-Resistant Campylobacter Species From Diarrheal Patients and Poultry Meat in Shanghai, China.
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DOI：
10.3389/fmicb.2018.01642
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发表时间：
2018
影响因子：
5.2
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Du Y;Wang C;Ye Y;Liu Y;Wang A;Li Y;Zhou X;Pan H;Zhang J;Xu X
对治疗嗜热弯曲杆菌感染的首选抗菌剂的新出现的耐药性正在成为对公共健康的严重威胁。用环丙沙星、四环素、庆大霉素、红霉素和克林霉素对548株腹泻患者和禽肉中分离的空肠弯曲杆菌和大肠埃希菌进行了药敏试验。其中151株(空肠弯曲菌32株，大肠埃希菌119株)为多重耐药菌株。对151株多重耐药菌株进行了PFGE分析，确定了103株PFGE基因分型。一些来自人和鸡的分离株属于相同的基因类型，表明这些克隆可能能够在人和鸡之间传播。对151株细菌进行了耐药基因鉴定。携带tet(O)、AADE、ermB和AADE-SAT4-APHA的菌株分别为148株(98%)、89株(58.9%)、31株(20.5%)和10株(6.6%)。几乎所有患者(n=150，99.3%)都有GyrA基因第86位密码子突变。其中56株(37.1%)存在23S rRNA A2075G点突变。CmeR-cmeABC间隔区的基因突变可能导致CmeABC多药外排泵的激活，本研究在空肠和大肠埃希菌中都发现了该间隔区的新序列类型。中国，本研究确定了上海地区腹泻患者和禽肉中分离的多药耐药弯曲杆菌耐药的遗传条件。
Emerging resistance to the antimicrobial agents of choice for treatment of thermophilic Campylobacter infections is becoming a serious threat to public health. In this study, 548 Campylobacter (372 C. jejuni and 176 C. coli) isolates from diarrheal patients and poultry meat were subjected for antibiotic susceptibility analysis to ciprofloxacin, tetracycline, gentamicin, erythromycin and clindamycin. Among them, 151 Campylobacter (32 C. jejuni and 119 C. coli) were identified as multidrug resistant isolates. PFGE analysis was performed on the 151 multidrug resistant isolates to determine their genetic relatedness, and 103 PFGE genotypes were determined. Some isolates from both human and chicken belonged to identical genotypes, indicating these clones might be able to spread between human and chicken. Antibiotic resistant genes of the 151 isolates were identified. The numbers of isolates carried tet (O), aadE, ermB, and aadE-sat4-aphA were 148 (98%), 89 (58.9%), 31 (20.5%), and 10 (6.6%), respectively. Almost all (n = 150, 99.3%) had gyrA mutation at codon 86. And the 23s rRNA A2075G point mutation was found in 56 (37.1%) isolates. Gene mutations at the cmeR-cmeABC intergenic region may lead to the activation of CmeABC multidrug efflux pump, and in this study novel sequence types of the intergenic region were identified in both C. jejuni and C. coli. This study determined the genetic prerequisites for antibiotic resistance of multidrug resistant Campylobacter isolates from diarrheal patients and poultry meat in Shanghai, China.
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影响因子：
5.2
作者：
Silva J;Leite D;Fernandes M;Mena C;Gibbs PA;Teixeira P
通讯作者：
Teixeira P
影响因子：
5.2
作者：
Florez-Cuadrado D;Ugarte-Ruiz M;Meric G;Quesada A;Porrero MC;Pascoe B;Sáez-Llorente JL;Orozco GL;Domínguez L;Sheppard SK
通讯作者：
Sheppard SK
影响因子：
--
作者：
Wieczorek K;Osek J
通讯作者：
Osek J
影响因子：
4.2
作者：
Zhang T;Luo Q;Chen Y;Li T;Wen G;Zhang R;Luo L;Lu Q;Ai D;Wang H;Shao H
通讯作者：
Shao H
影响因子：
4.2
作者：
Zhang T;Dong J;Cheng Y;Lu Q;Luo Q;Wen G;Liu G;Shao H
通讯作者：
Shao H