An Efficient Micropropagation System for Morinda officinalis How. (Rubiaceae), an Endangered Medicinal Plant
An Efficient Micropropagation System for Morinda officinalis How. (Rubiaceae), an Endangered Medicinal Plant
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巴戟天的高效微繁殖系统如何。
DOI：
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发表时间：
2015-11
影响因子：
1.2
通讯作者：
H. He
中科院分区：
文献类型：
--
作者：
Zh. Ch. Deng;H. Jin;H. He
本工作为珍稀濒危药用植物巴戟天建立了一套高效的直接器官发生再生繁殖体系。研究了外植体类型对不定芽诱导的影响，植物生长调节剂对不定芽增殖和伸长的影响，以及生根能力的影响。在不同类型的外植体中，以茎尖和节茎段为外植体的直接增殖是成功的，在适宜的培养基上培养8周后，95%左右的外植体每个外植体产生约5个不定芽。另一方面，叶片和节间外植体不产生任何芽。细胞分裂素对芽的增殖作用最强的是6-苄基腺嘌呤。当6-苄基腺嘌呤的浓度为1-2.0 mg L~(-1)时，可获得较高的平均枝数(每个外植体5个枝条)。将不定芽转移到含L 2.0~3.0 mg-1赤霉酸-3的Murashige和Skoog基本培养基上，在2周内获得了满意的芽伸长。在含L-1吲哚-3-丁酸0.2 mg的半强度Murashige+Skoog培养基上培养3周，生根率达100%。试管苗在温室驯化后移栽大田，成活率达90%。
The present work developed an efficient plant regeneration and propagation system via direct organogenesis for Morinda officinalis How., a valuable endangered medicinal plant. The effects of explant types on shoot induction, plant growth regulators on shoot proliferation and elongation and the subsequent rooting ability of shoots were examined. Among the various types of explant, direct shoot proliferation was successfully achieved from shoot tips and nodal stem segments with around 95% of the explants producing approximately 5 shoots per explant after 8 weeks of culture on optimal medium. On the other hand, leaf and internodal explants did not produce any shoots. The most effective cytokinin on shoot proliferation was 6-benzyladenine. When the concentration of 6benzyladenine was at 1.0-2.0 mg L -1 , a high mean shoot number (about 5 shoots per explant) was achieved. Shoot elongation was obtained satisfactorily by transferring the shoots to Murashige and Skoog basal media containing 2.0-3.0 mg L -1 gibberellic acid-3 within 2 weeks. Rooting was 100% on half-strength Murashige and Skoog medium containing 0.2 mg L -1 indole-3-butyric acid after 3 weeks of culture. The plantlets were acclimatized in the greenhouse and subsequently transferred to the field with 90% survival rate.
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影响因子：
5.9
作者：
Singh, Jayanti;Tiwari, Kavindra Nath
通讯作者：
Tiwari, Kavindra Nath
影响因子：
--
作者：
W. Tefera;S. Wannakrairoj
通讯作者：
W. Tefera;S. Wannakrairoj
影响因子：
1.2
作者：
M. Shatnawi
通讯作者：
M. Shatnawi
影响因子：
2.4
作者：
Faisal, Mohd.;Ahmad, Naseem;Anis, Mohammad
通讯作者：
Anis, Mohammad
影响因子：
6.2
作者：
Prakash Lakshmanan;C. Lee;C. Goh
通讯作者：
Prakash Lakshmanan;C. Lee;C. Goh