Degradation of Multiple Peptides by Microcystin-Degrader Paucibacter toxinivorans (2C20).
Degradation of Multiple Peptides by Microcystin-Degrader Paucibacter toxinivorans (2C20).
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DOI：
10.3390/toxins13040265
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发表时间：
2021-04-08
期刊：
影响因子：
4.2
通讯作者：
Edwards C
中科院分区：
文献类型：
--
作者：
Santos AA;Soldatou S;de Magalhães VF;Azevedo SMFO;Camacho-Muñoz D;Lawton LA;Edwards C
由于在不同国家应用的传统饮用水处理在消除潜在有毒的蓝藻肽方面效率低下，因此已经研究了许多细菌作为生物过滤器的替代物来去除微囊藻毒素（MC）。在此，我们评价了在7天内由噬毒少杆菌不仅降解MC变体（-LR/DM-LR/-RR/-LF/-YR），而且降解非MC肽（鱼腥藻肽A/B、气环酰胺A/D）。我们还评估了在肽混合物中MC-LR的降解速率，所有肽都进行了测试，并且存在M。铜绿假单胞菌粗提物。此外，生物降解进行了评估的非蓝藻肽具有不同的化学结构，如环孢菌素A，（Glu 1）-纤维蛋白肽-B，亮氨酸脑啡肽，催产素。当单独添加氰肽时，除MC-LR和-RR分别降低约85%和90%外，食毒假单胞菌在7天内降解了它们（99%）。与单个化合物相比，肽混合物中MC-LR的降解速率降低，然而，在微囊藻提取物存在下，其降解得相当快（3天）。值得注意的是，生物降解速率下降的混合物中的所有MC，而非MC肽立即降解。UPLC-QTOF-MS/MS使我们能够鉴定MC-LR和MC-YR的两种线性生物降解产物，以及MC-LF的一种。此外，P. toxinivorans证明了非蓝藻肽的完全降解，催产素除外，其中约50%在7天后保留。因此，尽管先前将P. toxinivorans鉴定为MC-降解剂，但它也在一系列条件下降解广泛的肽，这可以优化为用于水处理的潜在生物工具。
Since conventional drinking water treatments applied in different countries are inefficient at eliminating potentially toxic cyanobacterial peptides, a number of bacteria have been studied as an alternative to biological filters for the removal of microcystins (MCs). Here, we evaluated the degradation of not only MCs variants (-LR/DM-LR/-RR/-LF/-YR), but also non-MCs peptides (anabaenopeptins A/B, aerucyclamides A/D) by Paucibacter toxinivorans over 7 days. We also evaluated the degradation rate of MC-LR in a peptide mix, with all peptides tested, and in the presence of M. aeruginosa crude extract. Furthermore, biodegradation was assessed for non-cyanobacterial peptides with different chemical structures, such as cyclosporin A, (Glu1)-fibrinopeptide-B, leucine-enkephalin, and oxytocin. When cyanopeptides were individually added, P. toxinivorans degraded them (99%) over 7 days, except for MC-LR and -RR, which decreased by about 85 and 90%, respectively. The degradation rate of MC-LR decreased in the peptide mix compared to an individual compound, however, in the presence of the Microcystis extract, it was degraded considerably faster (3 days). It was noted that biodegradation rates decreased in the mix for all MCs while non-MCs peptides were immediately degraded. UPLC–QTOF–MS/MS allowed us to identify two linear biodegradation products for MC-LR and MC-YR, and one for MC-LF. Furthermore, P. toxinivorans demonstrated complete degradation of non-cyanobacterial peptides, with the exception of oxytocin, where around 50% remained after 7 days. Thus, although P. toxinivorans was previously identified as a MC-degrader, it also degrades a wide range of peptides under a range of conditions, which could be optimized as a potential biological tool for water treatment.
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影响因子：
6.6
作者：
Kohler, Esther;Grundler, Verena;Blom, Judith F.
通讯作者：
Blom, Judith F.
影响因子：
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作者：
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作者：
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作者：
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作者：
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