Bulk pollen pollination in maize for efficient construction of introgression populations with high genome coverage
Bulk pollen pollination in maize for efficient construction of introgression populations with high genome coverage
复制标题
玉米中的大量花粉授粉可有效构建具有高基因组覆盖度的渗入群体
DOI:
10.1111/pbr.12684
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发表时间:
2019-06
期刊:
影响因子:
2
通讯作者:
Hongwei Zhang
中科院分区:
文献类型:
--
作者:
Hongwei Zhang
Introgression populations consist of a set of introgression lines or families, constructed by continuous backcrossing to the recurrent parent, while carrying a limited number of chromosome segments from a donor parent in their genomes. Increasing the genome coverage is an important aim when constructing introgression population. In this study, we proposed bulk pollen pollination (BPP) method and used it to increase the genome coverage of a maize introgression population. The results showed that the genome coverage of the introgression population constructed using BPP method reached 100% at BC3generation, which accorded with the simulation result. The BPP‐based BC3F1:2population could identify most quantitative trait loci (QTL) detected using the F2:3population, especially major QTL. Simulation analysis showed that the genome coverage of introgression population increased with the increase of population size and the number of bulked plants, and decreased with the increase of backcross generation. Our results proved the reliability of the BPP‐based introgression population in increasing genome coverage and detecting QTL, and provided references for constructing high‐coverage introgression populations.
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影响因子:
5.6
作者:
Liu X;Han F;Kong C;Fang Z;Yang L;Zhang Y;Zhuang M;Liu Y;Li Z;Lv H
通讯作者:
Lv H
影响因子:
12.3
作者:
Bauer E;Falque M;Walter H;Bauland C;Camisan C;Campo L;Meyer N;Ranc N;Rincent R;Schipprack W;Altmann T;Flament P;Melchinger AE;Menz M;Moreno-González J;Ouzunova M;Revilla P;Charcosset A;Martin OC;Schön CC
通讯作者:
Schön CC
影响因子:
3.3
作者:
Falque, M;Décousset, L;Murigneux, A
通讯作者:
Murigneux, A
影响因子:
5.4
作者:
Eduardo, I;Arús, P;Monforte, AJ
通讯作者:
Monforte, AJ
影响因子:
3.1
作者:
Reinke, Russell;Kim, Suk-Man;Kim, Bo-Kyeong
通讯作者:
Kim, Bo-Kyeong