Bulk pollen pollination in maize for efficient construction of introgression populations with high genome coverage

Bulk pollen pollination in maize for efficient construction of introgression populations with high genome coverage
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玉米中的大量花粉授粉可有效构建具有高基因组覆盖度的渗入群体

DOI:
10.1111/pbr.12684
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发表时间:
2019-06
期刊:
影响因子:
2
通讯作者:
Hongwei Zhang
Hongwei Zhang
中科院分区:
农林科学3区
文献类型:
--
作者:
Hongwei Zhang

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基因渗入群体由一组基因渗入系或家族组成,通过与轮回亲本的连续回交构建,同时在其基因组中携带来自供体亲本的有限数量的染色体片段。增加基因组覆盖度是构建基因渗入群体时的一个重要目标。在这项研究中,我们提出了批量花粉授粉(BPP)方法,并用它来增加玉米渗入群体的基因组覆盖度。结果表明,利用BPP方法构建的渗入群体在BC3代时基因组覆盖率达到100%,与模拟结果相符。基于 BPP 的 BC3F1:2 群体可以识别使用 F2:3 群体检测到的大多数数量性状位点 (QTL),尤其是主要 QTL。模拟分析表明,基因渗入群体的基因组覆盖度随着群体规模和散装植株数量的增加而增加,随着回交代数的增加而减少。我们的结果证明了基于BPP的基因渗入群体在增加基因组覆盖度和检测QTL方面的可靠性,为构建高覆盖度的渗入群体提供了参考。
Introgression populations consist of a set of introgression lines or families, constructed by continuous backcrossing to the recurrent parent, while carrying a limited number of chromosome segments from a donor parent in their genomes. Increasing the genome coverage is an important aim when constructing introgression population. In this study, we proposed bulk pollen pollination (BPP) method and used it to increase the genome coverage of a maize introgression population. The results showed that the genome coverage of the introgression population constructed using BPP method reached 100% at BC3generation, which accorded with the simulation result. The BPP‐based BC3F1:2population could identify most quantitative trait loci (QTL) detected using the F2:3population, especially major QTL. Simulation analysis showed that the genome coverage of introgression population increased with the increase of population size and the number of bulked plants, and decreased with the increase of backcross generation. Our results proved the reliability of the BPP‐based introgression population in increasing genome coverage and detecting QTL, and provided references for constructing high‐coverage introgression populations.
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