Single-cell bioelectrical impedance platform for monitoring cellular response to drug treatment.
Single-cell bioelectrical impedance platform for monitoring cellular response to drug treatment.
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DOI：
10.1088/1478-3975/8/1/015006
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发表时间：
2011-02
期刊：
影响因子：
2
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Asphahani F;Wang K;Thein M;Veiseh O;Yung S;Xu J;Zhang M
细胞对化学或生物试剂的响应在其阻抗变化方面是实时的，这是一种有用的机制，可以用于各种各样的生物医学和环境应用。使用基于单细胞的分析平台可能是获得更灵敏的细胞阻抗测量的有效方法，特别是在预期阻抗仅发生微小变化的应用中。在这里，我们报告的芯片上的细胞阻抗生物传感器的发展与两种类型的电极，主机个别细胞和细胞群体，分别研究其在检测细胞反应的功效。通过配体介导的天然细胞粘附，在单细胞和多细胞电极上对人胶质母细胞瘤（U87 MG）细胞进行图案化。我们比较研究了两种类型的电极上的这些癌细胞如何响应离子通道抑制剂氯毒素（CTX），在它们的形状变化和阻抗变化方面，以利用基于单细胞的系统的精细可检测性。容纳单细胞的检测电极显示出真实的阻抗信号的显著降低，而容纳汇合单层细胞的电极显示出很少或没有阻抗变化。当单细胞电极用不同剂量的CTX处理时，观察到剂量依赖性的阻抗变化。这使我们能够确定这种特定治疗所需的有效剂量。我们的研究表明，这种单细胞阻抗系统可能作为一个有用的分析工具，生物医学应用，如环境毒素检测和药物评价。
The response of cells to a chemical or biological agent in terms of their impedance changes in real-time is a useful mechanism that can be utilized for a wide variety of biomedical and environmental applications. The use of a single-cell based analytical platform could be an effective approach to acquiring more sensitive cell impedance measurements, particularly in applications where only diminutive changes in impedance are expected. Here, we report the development of an on-chip cell impedance biosensor with two types of electrodes that hosts individual cells and cell populations, respectively, to study its efficacy in detecting cellular response. Human glioblastoma (U87MG) cells were patterned on single- and multi-cell electrodes through ligand-mediated natural cell adhesion. We comparatively investigated how these cancer cells on both types of electrodes respond to an ion channel inhibitor, chlorotoxin (CTX), in terms of their shape alternations and impedance changes to exploit the fine detectability of the single-cell based system. The detecting electrodes hosting single cells exhibited a significant reduction in the real impedance signal, while electrodes hosting confluent monolayer of cells showed little to no impedance change. When single-cell electrodes were treated with CTX of different doses, a dose-dependent impedance change was observed. This enables us to identify the effective dose needed for this particular treatment. Our study demonstrated that this single-cell impedance system may potentially serve as a useful analytical tool for biomedical applications such as environmental toxin detection and drug evaluation.
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影响因子：
2.7
作者：
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