Untangling the influences of voluntary running, environmental complexity, social housing and stress on adult hippocampal neurogenesis.
Untangling the influences of voluntary running, environmental complexity, social housing and stress on adult hippocampal neurogenesis.
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DOI：
10.1371/journal.pone.0086237
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Fernandes KJ
中科院分区：
文献类型：
--
作者：
Grégoire CA;Bonenfant D;Le Nguyen A;Aumont A;Fernandes KJ
丰富环境（EE）对大脑生理学有着强大的影响，并被广泛用作实验和治疗工具。典型的EE范式是多因素的，纳入体育锻炼，环境复杂性，社会互动和压力的元素，但这些变量的具体贡献还没有使用传统的住房范式分离。在这里，我们评估了这些个体变量对成年海马神经发生的影响，通过使用一种新的“交替EE”范式。连续4周，成年雄性CD 1小鼠每天在两个丰富的环境之间交替;通过比较两个环境中不同的组，可以解决EE变量的个体和组合效应。交替EE范式显示：（1）每周3天的自主跑步足以增加海马神经发生的有丝分裂期和有丝分裂后阶段，证实了运动的核心重要性;（2）复杂的环境（包括社会互动和旋转的无生命物体）对神经发生本身没有影响，但增强去极化诱导的c-Fos表达（归因于社会互动）和缓冲应激诱导的血浆皮质酮水平（归因于无生命的物体）;（3）无论是社会隔离，群体住房，也没有长期增加的血浆皮质酮水平对神经发生有长期的影响。对小鼠品系、处理和运行装置类型进行了检测，并排除了潜在混杂因素。这些研究结果提供了宝贵的见解成人神经发生的关键EE变量的相对影响，这种“交替EE”范式是一个有用的工具，探索个别EE变量的神经可塑性机制的贡献。
Environmental enrichment (EE) exerts powerful effects on brain physiology, and is widely used as an experimental and therapeutic tool. Typical EE paradigms are multifactorial, incorporating elements of physical exercise, environmental complexity, social interactions and stress, however the specific contributions of these variables have not been separable using conventional housing paradigms. Here, we evaluated the impacts of these individual variables on adult hippocampal neurogenesis by using a novel “Alternating EE” paradigm. For 4 weeks, adult male CD1 mice were alternated daily between two enriched environments; by comparing groups that differed in one of their two environments, the individual and combinatorial effects of EE variables could be resolved. The Alternating EE paradigm revealed that (1) voluntary running for 3 days/week was sufficient to increase both mitotic and post-mitotic stages of hippocampal neurogenesis, confirming the central importance of exercise; (2) a complex environment (comprised of both social interactions and rotated inanimate objects) had no effect on neurogenesis itself, but enhanced depolarization-induced c-Fos expression (attributable to social interactions) and buffered stress-induced plasma corticosterone levels (attributable to inanimate objects); and (3) neither social isolation, group housing, nor chronically increased levels of plasma corticosterone had a prolonged impact on neurogenesis. Mouse strain, handling and type of running apparatus were tested and excluded as potential confounding factors. These findings provide valuable insights into the relative effects of key EE variables on adult neurogenesis, and this “Alternating EE” paradigm represents a useful tool for exploring the contributions of individual EE variables to mechanisms of neural plasticity.
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影响因子：
82.9
作者：
Eriksson, PS;Perfilieva, E;Gage, FH
通讯作者：
Gage, FH
影响因子：
3.5
作者：
Clark, Peter J.;Brzezinska, Weronika J.;Puchalski, Emily K.;Krone, David A.;Rhodes, Justin S.
通讯作者：
Rhodes, Justin S.
影响因子：
5.5
作者：
HUBEL, DH;WIESEL, TN
通讯作者：
WIESEL, TN
影响因子：
10.6
作者：
Czéh, B;Welt, T;Keck, ME
通讯作者：
Keck, ME
DOI：
10.1073/pnas.95.6.3168
发表时间：
1998-03-17
影响因子：
11.1
作者：
Gould, E;Tanapat, P;Fuchs, E
通讯作者：
Fuchs, E