Simultaneous enhancement of photothermal stability and gene delivery efficacy of gold nanorods using polyelectrolytes.
Simultaneous enhancement of photothermal stability and gene delivery efficacy of gold nanorods using polyelectrolytes.
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DOI：
10.1021/nn900947a
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发表时间：
2009-10-27
期刊：
影响因子：
17.1
通讯作者：
Rege K
中科院分区：
文献类型：
--
作者：
Huang HC;Barua S;Kay DB;Rege K
纳米颗粒在水性介质中聚集的倾向阻碍了它们在生物医学应用中的有效使用。金纳米棒（GNRs）已经被研究作为治疗剂、成像剂和诊断剂。我们报告说，化学生成的金纳米棒在生物相关介质中迅速聚集。在金纳米棒上沉积纳米多层膜增强了这些纳米颗粒的稳定性至少长达四周。分散体的聚乙烯（PE）-金纳米棒组装体（PE-GNRs）表现出稳定的类Arrhenius光热反应，这是利用体外高温消融前列腺癌细胞。亚毒性浓度的PE-GNR组件也用于将外源质粒DNA递送至前列腺癌细胞。PE-GNRs的基础上，最近在我们的实验室中合成的阳离子型聚乙烯亚胺，目前的标准聚合物介导的基因传递相比，表现出更高的转染效率和更低的细胞毒性。我们的研究结果表明，明智的生物相容性聚电解质工程导致多功能的金纳米棒为基础的组件，结合联合收割机高稳定性和低细胞毒性与光热消融，基因传递，在一个单一的平台上的光学成像能力。
The propensity of nanoparticles to aggregate in aqueous media hinders their effective use in biomedical applications. Gold nanorods (GNRs) have been investigated as therapeutics, imaging agents, and diagnostics. We report that chemically generated gold nanorods rapidly aggregate in biologically relevant media. Depositing polyelectrolyte multilayers on gold nanorods enhanced the stability of these nanoparticles for at least up to four weeks. Dispersions of polyelectrolyte (PE)-gold nanorod assemblies (PE-GNRs) demonstrate a stable Arrhenius-like photothermal response, which was exploited for the hyperthermic ablation of prostate cancer cells in vitro. Sub-toxic concentrations of PE-GNR assemblies were also employed for delivering exogenous plasmid DNA to prostate cancer cells. PE-GNRs based on a cationic polyelectrolyte recently synthesized in our laboratory demonstrated higher transfection efficacy and lower cytotoxicity compared to those based on polyethyleneimine, a current standard for polymer-mediated gene delivery. Our results indicate that judicious engineering of biocompatible polyelectrolytes leads to multifunctional gold nanorod-based assemblies that combine high stability and low cytotoxicity with photothermal ablation, gene delivery, and optical imaging capabilities on a single platform.
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影响因子：
10.8
作者：
Durr, Nicholas J.;Larson, Timothy;Ben-Yakar, Adela
通讯作者：
Ben-Yakar, Adela
影响因子：
3.9
作者：
Huff, Terry B.;Hansen, Matthew N.;Wei, Alexander
通讯作者：
Wei, Alexander
影响因子：
10.8
作者：
Huang, Xiaohua;El-Sayed, Ivan H.;El-Sayed, Mostafa A.
通讯作者：
El-Sayed, Mostafa A.
影响因子：
4.3
作者：
Dhar, Sheetal;Reddy, E. Maheswara;Prasad, B. L. V.
通讯作者：
Prasad, B. L. V.
影响因子：
10.8
作者：
El-Sayed, IH;Huang, XH;El-Sayed, MA
通讯作者：
El-Sayed, MA