Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue.
Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue.
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DOI：
10.1038/s41536-022-00270-3
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发表时间：
2023-01-06
影响因子：
7.2
通讯作者：
Higgins, Claire A. A.
中科院分区：
文献类型：
--
作者：
Plotczyk, Magdalena;Jimenez, Francisco;Limbu, Summik;Boyle, Colin J. J.;Ovia, Jesse;Almquist, Benjamin D. D.;Higgins, Claire A. A.
尽管皮肤瘢痕形成对患者和医疗保健系统有重大影响，但缺乏预防瘢痕形成的策略，更不用说重塑成熟瘢痕的方法了。在这里，我们采取了一种独特的方法，灵感来自于健康的毛发皮肤在毛囊的定期循环过程中如何进行生理重塑。在这项初步临床研究中，我们测试了移植到人类疤痕中的毛囊是否可以促进组织再生并积极重塑纤维化组织，类似于它们重塑健康皮肤的方式。我们收集了全层皮肤活检，并比较了移植前后纤维化组织的形态学和转录特征。我们发现，毛囊移植诱导表皮厚度，表皮-真皮交界处，真皮细胞密度和血管密度的交错增加。I型胶原蛋白纤维的重塑减少了总胶原蛋白分数，粗纤维的比例，以及它们的对齐。与这些形态学变化一致，我们发现瘢痕的细胞因子环境发生了变化，并对促纤维化因子TGFβ1、IL-13和IL-6产生了长期抑制作用。我们的研究结果表明，生长期毛囊可以减弱纤维化表型，为开发再生方法重塑成熟疤痕提供了新的见解。
Despite the substantial impact of skin scarring on patients and the healthcare system, there is a lack of strategies to prevent scar formation, let alone methods to remodel mature scars. Here, we took a unique approach inspired by how healthy hairbearing skin undergoes physiological remodelling during the regular cycling of hair follicles. In this pilot clinical study, we tested if hair follicles transplanted into human scars can facilitate tissue regeneration and actively remodel fibrotic tissue, similar to how they remodel the healthy skin. We collected full-thickness skin biopsies and compared the morphology and transcriptional signature of fibrotic tissue before and after transplantation. We found that hair follicle tranplantation induced an increase in the epidermal thickness, interdigitation of the epidermal-dermal junction, dermal cell density, and blood vessel density. Remodelling of collagen type I fibres reduced the total collagen fraction, the proportion of thick fibres, and their alignment. Consistent with these morphological changes, we found a shift in the cytokine milieu of scars with a long-lasting inhibition of pro-fibrotic factors TGFβ1, IL13, and IL-6. Our results show that anagen hair follicles can attenuate the fibrotic phenotype, providing new insights for developing regenerative approaches to remodel mature scars.
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影响因子：
23.9
作者：
Joost, Simon;Annusver, Karl;Kasper, Maria
通讯作者：
Kasper, Maria
DOI：
10.1111/bjd.16255
发表时间：
2018-08
期刊：
The British journal of dermatology
影响因子：
--
作者：
Ghetti M;Topouzi H;Theocharidis G;Papa V;Williams G;Bondioli E;Cenacchi G;Connelly JT;Higgins CA
通讯作者：
Higgins CA
影响因子：
5.8
作者：
Kiani MT;Higgins CA;Almquist BD
通讯作者：
Almquist BD
影响因子：
9.7
作者：
Grant, C. A.;Twigg, P. C.;Tobin, D. J.
通讯作者：
Tobin, D. J.
影响因子：
--
作者：
HANSEN, LS;COGGLE, JE;CHARLES, MW
通讯作者：
CHARLES, MW