Cell type-specific responses to wingless, hedgehog and decapentaplegic are essential for patterning early eye-antenna disc in Drosophila.
Cell type-specific responses to wingless, hedgehog and decapentaplegic are essential for patterning early eye-antenna disc in Drosophila.
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DOI：
10.1371/journal.pone.0121999
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Cho KO
中科院分区：
文献类型：
--
作者：
Won JH;Tsogtbaatar O;Son W;Singh A;Choi KW;Cho KO
果蝇眼球触角成像盘(EAD)是一个扁平的双层上皮囊，大多数头部结构都是从这个囊中衍生出来的。分泌型形态原如Wingless(Wg)、Hedgehog(HH)和Decapentaplegic(DPP)在EAD的早期模式形成中起重要作用，但其潜在机制在很大程度上仍不清楚。为了了解这些形态原在早期幼虫EAD中的作用，我们使用了WG-LacZ和DPP-Gal4标记来检测野生型和突变型EADS。我们发现，孵化后即刻的eAD呈新月形，Bolwig的神经位于腹侧边缘，表明它由背侧区域组成。在随后的步骤中，DPP在Bolwig神经沿线的细胞中转录诱导，紧随其后的是腹侧结构域的快速增长。Wg和HH都是形成腹侧结构域所必需的。WG对整个EAD的生长至关重要，但HH仅在背侧区域对细胞分裂是必要的。在腹侧区域，HH调控DPP转录。基于这些数据，我们认为在一龄幼虫eAD中表达Wg、DPP或HH的不同细胞群之间的信号对于eAD的协调生长和模式是至关重要的。
The Drosophila eye-antenna imaginal disc (ead) is a flattened sac of two-layered epithelia, from which most head structures are derived. Secreted morphogens like Wingless (Wg), Hedgehog (Hh), and Decapentaplegic (Dpp) are important for early patterning of ead, but the underlying mechanisms are still largely unknown. To understand how these morphogens function in the ead of early larval stages, we used wg-LacZ and dpp-Gal4 markers for the examination of wild-type and mutant eads. We found that the ead immediately after hatching was crescent-shaped with the Bolwig’s nerve at the ventral edge, suggesting that it consists of dorsal domain. In a subsequent step, transcriptional induction of dpp in the cells along the Bolwig’s nerve was followed by rapid growth of the ventral domain. Both Wg and Hh were required for the formation of the ventral domain. Wg was crucial for the growth of the entire ead, but Hh was essential for cell division only in the dorsal domain. In the ventral domain, Hh regulated dpp transcription. Based on these data, we propose that signaling among distinct groups of cells expressing Wg, Dpp, or Hh in the ead of the first-instar larvae are critical for coordinated growth and patterning of ead.
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DOI：
10.1073/pnas.89.5.1919
发表时间：
1992-03-01
影响因子：
11.1
作者：
KASSIS, JA;NOLL, E;PERRIMON, N
通讯作者：
PERRIMON, N
影响因子：
10.5
作者：
Lee, T;Hacohen, N;Montell, DJ
通讯作者：
Montell, DJ
影响因子：
2.7
作者：
Blanco, Jorge;Seimiya, Makiko;Gehring, Walter J.
通讯作者：
Gehring, Walter J.
影响因子：
56.9
作者：
Brook, WJ;Cohen, SM
通讯作者：
Cohen, SM
影响因子：
64.5
作者：
Gibson, MC;Schubiger, G
通讯作者：
Schubiger, G