Changes in the Nasal Microbiota of Pigs Following Single or Co-Infection with Porcine Reproductive and Respiratory Syndrome and Swine Influenza A Viruses.
Changes in the Nasal Microbiota of Pigs Following Single or Co-Infection with Porcine Reproductive and Respiratory Syndrome and Swine Influenza A Viruses.
复制标题
DOI：
10.3390/pathogens10101225
复制
发表时间：
2021-09-22
期刊：
影响因子：
--
通讯作者：
Tchilian E
中科院分区：
文献类型：
--
作者：
Chrun T;Leng J;La Ragione RM;Graham SP;Tchilian E
宿主-微生物区系的相互作用在形成免疫反应方面很重要，免疫反应可能会影响病原体感染的结果。然而，大多数研究都集中在肠道微生物区系及其与疾病结局的可能关系上，而对鼻部微生物区系和呼吸道病原体感染的作用研究较少。在这里，我们检测了实验感染猪繁殖与呼吸综合征病毒2(PRRSV-2)、猪流感A H3N 2病毒(H3N 2)或这两种病毒后，猪鼻微生物区系组成的变化。从鼻拭子中提取DNA进行16S rRNA测序，以研究鼻腔微生物区系的组成。细菌丰富度在所有组中波动，与未感染对照组相比，在感染PRRSV-2和H3N2的前5天，单独感染PRRSV-2和H3N2的猪的细菌丰富度略有下降。相反，在PRRSV-2/H3N2混合感染后，鼻腔细菌丰度保持相对稳定。PRRSV-2和H3N2单独或联合使用对细菌家族的丰度和分布有不同的影响。与PRRSV-2或H3N_2的单独或混合感染与菊科的扩大有关。H3N2病毒载量与菊科植物的相对丰度呈正相关。然而，需要进一步的机制研究来了解这些病毒感染后特定细菌家族变化的意义。
Host-microbiota interactions are important in shaping immune responses that have the potential to influence the outcome of pathogen infection. However, most studies have focused on the gut microbiota and its possible association with disease outcome, while the role of the nasal microbiota and respiratory pathogen infection has been less well studied. Here we examined changes in the composition of the nasal microbiota of pigs following experimental infection with porcine reproductive and respiratory syndrome virus 2 (PRRSV-2), swine influenza A H3N2 virus (H3N2) or both viruses. DNA extracted from nasal swabs were subjected to 16S rRNA sequencing to study the composition of the nasal microbiota. Bacterial richness fluctuated in all groups, with a slight reduction in pigs singly infected with PRRSV-2 and H3N2 during the first 5 days of infection compared to uninfected controls. In contrast, nasal bacterial richness remained relatively stable after PRRSV-2/H3N2 co-infection. PRRSV-2 and H3N2, alone or in combination differentially altered the abundance and distribution of bacterial families. Single and co-infection with PRRSV-2 or H3N2 was associated with the expansion of the Neisseriaceae family. A positive correlation between H3N2 viral load and the relative abundance of the Neisseriaceae was observed. However, further mechanistic studies are required to understand the significance of the changes in specific bacterial families following these viral infections.
登录
查看更多内容
影响因子：
4.6
作者：
Correa-Fiz F;Neila-Ibáñez C;López-Soria S;Napp S;Martinez B;Sobrevia L;Tibble S;Aragon V;Migura-Garcia L
通讯作者：
Migura-Garcia L
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
3.6
作者：
Bertho, Nicolas;Meurens, Francois
通讯作者：
Meurens, Francois
影响因子：
4.7
作者：
Pirolo M;Espinosa-Gongora C;Bogaert D;Guardabassi L
通讯作者：
Guardabassi L
影响因子：
3.3
作者：
Fablet, C.;Marois-Crehan, C.;Rose, N.
通讯作者：
Rose, N.