Agua Salud Alphavirus Infection, Dissemination and Transmission in Aedes aegypti Mosquitoes.
Agua Salud Alphavirus Infection, Dissemination and Transmission in Aedes aegypti Mosquitoes.
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DOI：
10.3390/v15051113
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发表时间：
2023-05-03
期刊：
影响因子：
--
通讯作者：
Altinli M
中科院分区：
文献类型：
--
作者：
Jagtap SV;Brink J;Frank SC;Badusche M;Leggewie M;Sreenu VB;Fuss J;Schnettler E;Altinli M
蚊子是许多重要的节肢动物传播病毒（虫媒病毒）的有效载体。除虫媒病毒外，还在蚊子中发现了昆虫特异性病毒（ISV）。ISV是在昆虫宿主中复制但不能在脊椎动物中感染和复制的病毒。在某些情况下，它们已被证明能干扰虫媒病毒的复制。尽管对ISV-虫媒病毒相互作用的研究有所增加，但ISV与其宿主的相互作用以及它们如何在自然界中维持仍然没有很好的了解。本研究通过不同的感染途径（经口感染、胸腔内注射）和传播途径，调查了Agua Salud甲病毒（ASALV）在埃及伊蚊体内的感染和传播情况。我们在这里表明，ASALV感染女性的Ae。埃及伊蚊，并在蚊子胸腔内或经口感染时复制。ASALV播散至不同组织，包括中肠、唾液腺和卵巢。然而，我们观察到大脑中的病毒载量高于唾液腺和尸体，表明对脑组织的向性。我们的研究结果表明，ASALV在成虫和幼虫阶段是水平传播的，虽然我们没有观察到垂直传播。了解ISV感染和传播动力学在Ae。埃及伊蚊及其传播途径可能有助于将ISV用作未来的虫媒病毒控制策略。
Mosquitoes are competent vectors for many important arthropod-borne viruses (arboviruses). In addition to arboviruses, insect-specific viruses (ISV) have also been discovered in mosquitoes. ISVs are viruses that replicate in insect hosts but are unable to infect and replicate in vertebrates. They have been shown to interfere with arbovirus replication in some cases. Despite the increase in studies on ISV–arbovirus interactions, ISV interactions with their hosts and how they are maintained in nature are still not well understood. In the present study, we investigated the infection and dissemination of the Agua Salud alphavirus (ASALV) in the important mosquito vector Aedes aegypti through different infection routes (per oral infection, intrathoracic injection) and its transmission. We show here that ASALV infects the female Ae. aegypti and replicates when mosquitoes are infected intrathoracically or orally. ASALV disseminated to different tissues, including the midgut, salivary glands and ovaries. However, we observed a higher virus load in the brain than in the salivary glands and carcasses, suggesting a tropism towards brain tissues. Our results show that ASALV is transmitted horizontally during adult and larval stages, although we did not observe vertical transmission. Understanding ISV infection and dissemination dynamics in Ae. aegypti and their transmission routes could help the use of ISVs as an arbovirus control strategy in the future.
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