Development of a high-performance open-source 3D bioprinter.
Development of a high-performance open-source 3D bioprinter.
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DOI：
10.1038/s41598-022-26809-4
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发表时间：
2022-12-31
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
3D打印在生物研究中的应用为组织工程界提供了一种将细胞和生物材料组织成复杂3D结构的方法。虽然存在许多商业生物打印平台，但它们都很昂贵，从5000美元到超过100万美元不等。这种高昂的进入成本阻止了许多实验室将3D生物打印纳入他们的研究。由于桌面塑料3D打印机的开源性质，另一种选择是将低成本的塑料打印机转换为生物打印机。已经描述了几种开源修改，但仍然需要一种用户友好的分步指南，用于使用具有验证性能的组件将热塑性打印机转换为生物打印机。在这里，我们使用struder 4注射泵和Duet 3D Duet 2 WiFi将低成本3D打印机FlashForge Finder转换为生物打印机，总成本不到900美元。我们证明，生物打印机的行程精度在所有三个轴上都优于35 µm，并通过打印正方形晶格胶原支架来量化保真度，平均误差小于2%。我们还通过使用胶原蛋白生物墨水打印人耳支架来展示临床成像数据的高保真再现。最后，为了最大限度地提高可访问性和可定制性，我们为生物打印机转换设计的所有组件都以开源3D模型的形式提供，并沿着提供了进一步修改生物打印机以适应其他用例的说明，从而为生物打印领域提供了全面的指南。
The application of 3D printing to biological research has provided the tissue engineering community with a method for organizing cells and biological materials into complex 3D structures. While many commercial bioprinting platforms exist, they are expensive, ranging from $5000 to over $1,000,000. This high cost of entry prevents many labs from incorporating 3D bioprinting into their research. Due to the open-source nature of desktop plastic 3D printers, an alternative option has been to convert low-cost plastic printers into bioprinters. Several open-source modifications have been described, but there remains a need for a user-friendly, step-by-step guide for converting a thermoplastic printer into a bioprinter using components with validated performance. Here we convert a low-cost 3D printer, the FlashForge Finder, into a bioprinter using our Replistruder 4 syringe pump and the Duet3D Duet 2 WiFi for total cost of less than $900. We demonstrate that the accuracy of the bioprinter’s travel is better than 35 µm in all three axes and quantify fidelity by printing square lattice collagen scaffolds with average errors less than 2%. We also show high fidelity reproduction of clinical-imaging data by printing a scaffold of a human ear using collagen bioink. Finally, to maximize accessibility and customizability, all components we have designed for the bioprinter conversion are provided as open-source 3D models, along with instructions for further modifying the bioprinter for additional use cases, resulting in a comprehensive guide for the bioprinting field.
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影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
19
作者：
Romanazzo, Sara;Molley, Thomas Gregory;Roohani, Iman
通讯作者：
Roohani, Iman
影响因子：
10
作者：
Seymour AJ;Shin S;Heilshorn SC
通讯作者：
Heilshorn SC
影响因子：
19
作者：
Hull, Sarah M.;Lindsay, Christopher D.;Heilshorn, Sarah C.
通讯作者：
Heilshorn, Sarah C.
定制的超低成本三维生物打印机支持细胞生长和分化。
影响因子：
5.7
作者：
Ioannidis K;Danalatos RI;Champeris Tsaniras S;Kaplani K;Lokka G;Kanellou A;Papachristou DJ;Bokias G;Lygerou Z;Taraviras S
通讯作者：
Taraviras S