Electrospraying Electrospun Nanofiber Segments into Injectable Microspheres for Potential Cell Delivery.
Electrospraying Electrospun Nanofiber Segments into Injectable Microspheres for Potential Cell Delivery.
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DOI：
10.1021/acsami.8b06386
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发表时间：
2018-08-01
影响因子：
9.5
通讯作者：
Xie J
中科院分区：
文献类型：
--
作者：
Boda SK;Chen S;Chu K;Kim HJ;Xie J
纳米纤维微球由于其可注射性和仿生性，在生物医学领域的应用引起了人们的极大关注。在此，我们首次报道了一种将电纺和电喷雾相结合来制备纳米纤维微球的新方法，并探索了它们在细胞治疗方面的潜在应用。将所需长度的电纺纳米纤维片段的水分散体电喷到液氮中，然后冷冻干燥和热处理，就可以形成纳米纤维微球。在电喷雾过程中，可以通过改变施加电压来控制微球的尺寸。得到了各种形貌的微球，包括固体微球、纳米纤维微球、多孔微球和纳米纤维微球以及中空纳米纤维微球。此外，通过对聚合物和无机生物活性玻璃纳米纤维的短分散体进行电喷涂，可以制备出广泛的聚合物和无机生物活性玻璃纳米纤维微球，这表明该方法具有广泛的适用性。与固体微球相比，纳米纤维微球在大鼠骨髓间充质干细胞中具有更高的细胞载体效率，并在原位形成了微组织样结构，当注入到微通道设备中时。此外，小鼠胚胎干细胞在纳米纤维微球上进行了神经分化，β-III-微管蛋白阳性染色和轴突生长表明。综上所述，我们开发了一种新的方法来生成可注射的纳米纤维微球，并提高了干细胞的活性和维持性，用于细胞治疗的潜在应用。
Nanofiber microspheres have attracted a lot of attention for biomedical applications because of their injectable and biomimetic properties. Herein, we report for the first time a new method for fabrication of nanofiber microspheres by combining electrospinning and electrospraying and explore their potential applications for cell therapy. Electrospraying of aqueous dispersions of electrospun nanofiber segments with desired length obtained by either cryocutting or homogenization into liquid nitrogen followed by freeze-drying and thermal treatment can form nanofiber microspheres. The microsphere size can be controlled by varying the applied voltage during the electrospray process. A variety of morphologies were achieved including solid, nanofiber, porous and nanofiber microspheres, and hollow nanofiber microspheres. Furthermore, a broad range of polymer and inorganic bioactive glass nanofiber-based nanofiber microspheres could be fabricated by electrospraying of their short nanofiber dispersions, indicating a comprehensive applicability of this method. A higher cell carrier efficiency of nanofiber microspheres as compared to solid microspheres was demonstrated with rat bone marrow-derived mesenchymal stem cells, along with the formation of microtissue-like structures in situ, when injected into microchannel devices. Also, mouse embryonic stem cells underwent neural differentiation on the nanofiber microspheres, indicated by positive staining of β-III-tubulin and neurite outgrowth. Taken together, we developed a new method for generating nanofiber microspheres that are injectable and have improved viability and maintenance of stem cells for potential application in cell therapy.
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影响因子：
4.9
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
6.2
作者：
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通讯作者：
Zhang, Lina