Local photo-polymer deposition-assisted fabrication of multilayer paper-based devices
Local photo-polymer deposition-assisted fabrication of multilayer paper-based devices
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多层纸基器件的局部光聚合物沉积辅助制造
DOI：
10.1016/j.snb.2020.128574
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Galanis P
中科院分区：
文献类型：
--
作者：
Galanis P
在本文中，我们报告了使用基于丙烯酸酯的局部负性光聚合物沉积技术来制造3D微流体纸基分析装置（3D-μ PAD），其中样品在横向和垂直方向上流过多孔材料的多个堆叠层。使用简单且廉价的制造方法，其基于在多孔纤维素纸基底上局部沉积光聚合物（沉积速度30 mm/s），随后暴露于激光源（最大功率为60 mW的405 nm的光纤耦合连续波）（扫描速度30 mm/s），以堆叠四层纤维素纸并制造3D多层μ PAD。通过这种技术，我们提供了一种途径来消除其他报道的方法在制造μ PAD期间的限制，例如相邻层之间需要多个复杂的对准以及使用额外的工具来确保层之间的充分接触。在这项研究中，我们证明了我们的四层3D-μPAD的实用性，同时检测三种分析物，即BSA，葡萄糖，亚硝酸盐掺入人工尿液和测试样品的pH值，通过一步比色测定，发现检测限为0.4 mg/mL的BSA，14.5 μg/mL的葡萄糖和2.5 μg/mL的亚硝酸盐。我们的3D-μPAD制造方法也可以适用于更复杂的分析测定，其中需要多个步骤才能用于即时诊断。
In this paper, we report on the use of a local acrylate-based negative photo-polymer deposition technique for the fabrication of 3D microfluidic paper-based analytical devices (3D-μPADs) where the sample flows in both the lateral and vertical directions through multiple stacked layers of porous materials. A simple and inexpensive manufacturing method was used, which is based on the local deposition of a photo-polymer (deposition speed 30 mm/s) on a porous cellulose paper substrate followed by the subsequent exposure (scanning speed 30 mm/s) to a laser source (fibre coupled continuous wave at 405 nm with maximum power of 60 mW), to stack four layers of cellulose paper and make 3D multilayer μPADs. With this technique, we provide a pathway to eliminate the limitations that other reported methods have during the fabrication of μPADs such as the need for multiple sophisticated alignments between adjoining layers and the use of additional tools to ensure adequate contact between the layers. In this study, we demonstrate the usefulness of our four-layer 3D-μPAD for simultaneous detection of three analytes, namely BSA, glucose, nitrite spiked in artificial urine and also the pH of the tested sample, through single step colorimetric assays with the limit of detection found at 0.4 mg/mL for BSA, 14.5 μg/mL for glucose and 2.5 μg/mL for nitrite. Our 3D-μPAD fabrication methodology can also be adapted in more complex analytical assays where multiple steps are needed for applications in point-of-care diagnostics.
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影响因子：
3.9
作者：
Renault, Christophe;Koehne, Jessica;Crooks, Richard M.
通讯作者：
Crooks, Richard M.
DOI：
10.1039/c4an01147c
发表时间：
2014-11-07
期刊：
The Analyst
影响因子：
--
作者：
Evans E;Gabriel EF;Benavidez TE;Tomazelli Coltro WK;Garcia CD
通讯作者：
Garcia CD
影响因子：
7.4
作者：
Rattanarat, Poomrat;Dungchai, Wijitar;Henry, Charles S.
通讯作者：
Henry, Charles S.
影响因子：
6.1
作者：
Channon RB;Nguyen MP;Scorzelli AG;Henry EM;Volckens J;Dandy DS;Henry CS
通讯作者：
Henry CS
影响因子：
11.4
作者：
Mani, Vigneshwaran;Kadimisetty, Karteek;Malla, Spundana;Joshi, Amit A.;Rusling, James F.
通讯作者：
Rusling, James F.