Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct.
Reduction in wound bioburden using a silver-loaded dissolvable microfilm construct.
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DOI：
10.1002/adhm.201300537
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发表时间：
2014-06
影响因子：
10
通讯作者：
Abbott, Nicholas L.
中科院分区：
文献类型：
--
作者：
Herron, Maggie;Agarwal, Ankit;Kierski, Patricia R.;Calderon, Diego F.;Teixeira, Leandro B. C.;Schurr, Michael J.;Murphy, Christopher J.;Czuprynski, Charles J.;McAnulty, Jonathan F.;Abbott, Nicholas L.
银是一种广泛使用的抗菌剂，但当浸渍在肉眼可见的敷料中时，它会污染伤口，可能导致组织毒性并可能抑制愈合。最近，我们报道了含有银纳米颗粒的聚合物纳米膜在银的载量和释放速率比传统敷料低100倍时表现出抗微生物活性。在这里，我们报告的复合微膜结构，提供了一种简便的方法来转移负载银的聚合物纳米膜到体内伤口的制造。该结构由负载银纳米颗粒的聚合物纳米薄膜制成，该薄膜与微米厚的聚乙烯醇（PVA）可溶性薄膜层压在一起。当放置在潮湿的伤口上时，PVA溶解，使载银纳米膜固定在伤口床上。在体外，固定化纳米膜在30天内从皮肤-真皮释放<1 μg cm-2/天的银，并且它们在24小时内杀死5 log 10 CFU的金黄色葡萄球菌。在小鼠中，伤口接种105 CFU S。当用银/纳米膜处理3天时，与未修饰的伤口相比，金黄色葡萄球菌呈现高达3log 10的更少的细菌负荷。在未污染的伤口中，银/纳米膜通过上皮再形成允许正常和完全的伤口闭合。我们的结论是，可溶性微膜结构可以克服与目前使用的银在伤口愈合的关键限制。
Silver is a widely used antimicrobial agent, yet when impregnated in macroscopic dressings, it stains wounds, can lead to tissue toxicity and can inhibit healing. Recently, we reported that polymeric nanofilms containing silver nanoparticles exhibit antimicrobial activity at loadings and release rates of silver that are 100x lower than conventional dressings. Here we report fabrication of composite microfilm constructs that provide a facile way to transfer the silver-loaded polymeric nanofilms onto wounds in vivo. The construct is fabricated from a silver nanoparticle-loaded polymeric nanofilm that is laminated with a micrometer-thick soluble film of polyvinylalcohol (PVA). When placed on a moist wound, the PVA dissolves, leaving the silver-loaded nanofilm immobilized on the wound-bed. In vitro, the immobilized nanofilms release <1 μg cm−2/day of silver over 30 days from skin-dermis and they kill 5 log10 CFUs of Staphylococcus aureus in 24 h. In mice, wounds inoculated with 105 CFU S. aureus presented up to 3 log10 less bacterial burden when treated with silver/nanofilms for 3 days, as compared to unmodified wounds. In uncontaminated wounds, silver/nanofilms allow normal and complete wound closure by re-epithelialization. We conclude that dissolvable microfilm constructs may overcome key limitations associated with current uses of silver in wound healing.
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影响因子：
2.9
作者：
Burd, Andrew;Kwok, Chi H.;Huang, Lin
通讯作者：
Huang, Lin
影响因子：
19
作者：
Fujie, Toshinori;Matsutani, Noriyuki;Takeoka, Shinji
通讯作者：
Takeoka, Shinji
影响因子：
2.2
作者：
Herigstad, B;Hamilton, M;Heersink, J
通讯作者：
Heersink, J
影响因子：
9
作者：
Guthrie KM;Agarwal A;Tackes DS;Johnson KW;Abbott NL;Murphy CJ;Czuprynski CJ;Kierski PR;Schurr MJ;McAnulty JF
通讯作者：
McAnulty JF
影响因子：
2.7
作者：
Hermans, Michel H.
通讯作者：
Hermans, Michel H.