Functional analysis of the ScAG and ScAGL11 MADS-box transcription factors for anthocyanin biosynthesis and bicolour pattern formation in Senecio cruentus ray florets.
Functional analysis of the ScAG and ScAGL11 MADS-box transcription factors for anthocyanin biosynthesis and bicolour pattern formation in Senecio cruentus ray florets.
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ScAG 和 ScAGL11 MADS-box 转录因子对千里光花色苷生物合成和双色图案形成的功能分析
DOI：
10.1093/hr/uhac071
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发表时间：
2022
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
作者：
金银花（Senecio cruentus）是一种纯色和双色的观赏植物，广泛用于园林绿化。花青素的生物合成影响灰孢的颜色模式。然而，花青素在金银花中的积累和分布机制尚不清楚。本研究以纯色和双色品种为研究对象，探讨了花青素合成和双色形成的分子机制。转录组和基因表达分析显示，在双色品种的白色区和无色区，ScCHS2、ScF3H1、ScDFR3、ScANS和ScbHLH17 5个基因被抑制。相比之下，两个MADS-box基因ScAG和ScAGL11在双色品种的无色区表达量显著增加。ScAG和ScAGL11定位于细胞核，并与双色性状共表达。进一步的功能分析证实，ScAG能抑制烟草花青素的积累。然而，病毒诱导的基因沉默（VIGS）实验表明，ScAG和ScAGL11的沉默增加了玉米叶片中花青素的含量。当ScAG和ScAGL11在头孢草中被沉默时，观察到类似的结果，伴随着较小的无色区域，特别是在ScAG/ScAGL11沉默的植物中。ScCHS2、ScDFR3和ScF3H1在沉默的金银花叶片和头状花序中表达增加。此外，酵母双杂交和双分子荧光互补实验表明，ScAG与ScAGL11相互作用。此外，ScAG直接抑制ScF3H1的转录，而ScAGL11通过分别结合其启动子抑制ScDFR3的表达。研究结果表明，ScAG和ScAGL11负向调控花青素在银穗子小花中的生物合成，其在银穗子小花中的差异表达影响双色花纹的出现。
Cineraria (Senecio cruentus) is an ornamental plant with pure colour and bicolour cultivars, widely used for landscaping. Anthocyanin biosynthesis influences coloration patterns in cineraria. However, how anthocyanins accumulate and distribute in cineraria is poorly understood. This study investigated the molecular mechanisms underlying anthocyanin biosynthesis and bicolour formation in cineraria using pure colour and bicolour cultivars. Transcriptome and gene expression analysis showed that five genes, ScCHS2, ScF3H1, ScDFR3, ScANS, and ScbHLH17, were inhibited in the white cultivar and colourless regions of bicolour cultivars. In contrast, two MADS-box genes, ScAG and ScAGL11, showed significantly higher expression in the colourless regions of bicolour cultivars. ScAG and ScAGL11 were localized in the nucleus and co-expressed with the bicolour trait. Further functional analysis verified that ScAG inhibits anthocyanin accumulation in tobacco (Nicotiana tabacum). However, virus-induced gene silencing (VIGS) experiments showed that silencing of ScAG and ScAGL11 increases anthocyanin content in cineraria leaves. Similar results were observed when ScAG and ScAGL11 were silenced in the cineraria capitulum, accompanied by the smaller size of the colourless region, specifically in the ScAG/ScAGL11-silenced plants. The expression of ScCHS2, ScDFR3, and ScF3H1 increased in silenced cineraria leaves and capitulum. Furthermore, yeast two-hybrid and bimolecular fluorescence complementation experiments demonstrated that ScAG interacts with ScAGL11. Moreover, ScAG directly inhibited the transcription of ScF3H1 while ScAGL11 inhibited ScDFR3 expression by binding to their promoters separately. The findings reported herein indicate that ScAG and ScAGL11 negatively regulate anthocyanin biosynthesis in cineraria ray florets, and their differential expression in ray florets influences the bicolour pattern appearance.
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影响因子：
5.6
作者：
Jin X;Huang H;Wang L;Sun Y;Dai S
通讯作者：
Dai S
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影响因子：
5.3
作者：
Kapazoglou A;Engineer C;Drosou V;Kalloniati C;Tani E;Tsaballa A;Kouri ED;Ganopoulos I;Flemetakis E;Tsaftaris AS
通讯作者：
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影响因子：
13.8
作者：
An, Jian-Ping;Wang, Xiao-Fei;Hao, Yu-Jin
通讯作者：
Hao, Yu-Jin
DOI：
10.1073/pnas.83.12.4428
发表时间：
1986-06-01
影响因子：
11.1
作者：
HORSCH, RB;KLEE, HJ
通讯作者：
KLEE, HJ
影响因子：
7.4
作者：
Jaakola, Laura;Poole, Mervin;Seymour, Graham B.
通讯作者：
Seymour, Graham B.