Fabrication of enzyme-responsive composite coating for the design of antibacterial surface
Fabrication of enzyme-responsive composite coating for the design of antibacterial surface
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用于抗菌表面设计的酶响应复合涂层的制备
DOI：
10.1007/s10856-018-6171-0
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Cai Kaiyong
中科院分区：
文献类型：
--
作者：
Liu Peng;Hao Yansha;Ding Yao;Yuan Zhang;Liu Yisi;Cai Kaiyong
本研究开发了一种具有银离子控制释放的细菌酶触发抗菌表面。首先，以抗坏血酸为还原剂，原位合成了壳聚糖-银纳米复合材料（Chi@Ag NPs）。通过透射电子显微镜、紫外-可见光谱、X射线衍射和X射线光电子能谱对Chi@Ag纳米粒子进行了表征。随后，将Chi@Ag纳米粒子与透明质酸（HA）通过层层自组装（LBL）法制备了抗菌复合涂层。通过扫描电镜、能谱仪和接触角测试等手段，证实了Chi@Ag NPs/HA复合涂层的成功构建。然后，通过电感耦合等离子体原子发射光谱法分析释放的银离子的量，这表明银离子从表面的释放可以由酶（例如透明质酸酶）触发。通过抑菌圈试验、细菌活力测定、抗菌率测定和细菌粘附观察等一系列体外抗菌试验，证明酶响应表面能抑制细菌的生长。总体而言，本研究为在各个领域的合成材料上制备抗菌表面提供了一种替代方法，对周围环境和人体的副作用最小。
In this study, a type of bacteria enzyme-triggered antibacterial surface with a controlled release of Ag ions was developed. Firstly, chitosan-silver nanocomposites (Chi@Ag NPs) were in situ synthesized via using ascorbic acid as reducing agent. Chi@Ag NPs were characterized by transmission electron microscopy, ultraviolet–visible spectroscopy, X-ray diffraction and X-ray photoelectron spectroscopy. Subsequently, Chi@Ag NPs and hyaluronic acid (HA) were used to fabricate antibacterial composite coating via Layer-by-Layer (LBL) self-assembly method. The successful construction of Chi@Ag NPs/HA composite coating was confirmed by scanning electron microscopy, energy dispersive spectroscopy and contact angle measurements, respectively. Then, the amount of released Ag ion was analyzed by inductively coupled plasma atomic emission spectrometry, which demonstrated that the release of Ag ions from the surface could be triggered by enzyme (e.g. hyaluronidase). A series of antibacterial tests in vitro, including zone of inhibition test, bacterial viability assay, antibacterial rate measurement and bacteria adhesion observation, demonstrated that the enzyme-responsive surface could inhibit the growth of bacteria. On the whole, this study provides an alternative approach for the fabrication of antibacterial surfaces on synthetic materials in various fields with the minimal side effects on surrounding environment and human body.
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DOI：
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期刊：
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影响因子：
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