Identification, localization, and primary structure of CAP-23, a particle-bound cytosolic protein of early development.
Identification, localization, and primary structure of CAP-23, a particle-bound cytosolic protein of early development.
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CAP-23的鉴定，定位和主要结构，这是一种早期发育的粒子结合的胞质蛋白。
DOI：
10.1083/jcb.111.6.3035
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发表时间：
1990-12
期刊：
影响因子：
--
通讯作者：
Caroni P
中科院分区：
文献类型：
--
作者：
Widmer F;Caroni P
我们报告的CAP-23，一种新的颗粒结合的细胞溶质蛋白与哺乳动物和鸟类组织中的发育细胞的鉴定。CAP-23在体外是纯化的蛋白激酶C（PKC）的底物，并且该蛋白在培养的细胞中以PMA敏感的方式被磷酸化，表明它是PKC的原位底物。分离编码鸡CAP-23的cDNA。推导的序列揭示了一个不寻常的氨基酸组成，惊人的相似，大鼠GAP-43，生长相关的神经元特异性PKC底物。CAP-23的进一步预测特征包括在Ser-6处的PKC磷酸化位点，以及碱性NH 2-和COOH-末端结构域的存在。CAP-23由约1.5kb的mRNA编码，其在鸡发育过程中的分布类似于相应的蛋白质。Southern印迹分析显示，在鸡基因组中存在一个单一的主要杂交物种。CAP-23在发育过程中的分布用蛋白质印迹法和组织切片上的免疫荧光法进行分析。在培养的细胞中，蛋白质似乎以规则的斑点图案分布在整个细胞表面下方。在早期鸡胚（E2）中，CAP-23存在于大多数（如果不是所有）细胞中。然后，蛋白质逐渐局限于某些发育中的组织和这些组织中的某些细胞。在大多数组织中，CAP-23水平在E15和E19之间低于检测限。在神经系统中发现了最高水平的蛋白质，其中CAP-23水平在E18左右达到峰值，并且在出生时仍然可以检测到升高的水平。
We report the identification of CAP-23, a novel particle-bound cytosolic protein associated with developing cells in both mammalian and avian tissues. CAP-23 was a substrate for purified protein kinase C (PKC) in vitro, and the protein was phosphorylated in a PMA-sensitive manner in cultured cells, indicating that it is a PKC substrate in situ. cDNA coding for chick CAP-23 was isolated. The deduced sequence revealed an unusual amino acid composition that strikingly resembled that of rat GAP-43, a growth-associated neuron-specific PKC substrate. Further predicted features of CAP-23 included a PKC phosphorylation site at Ser-6, and the presence of basic NH2- and COOH-terminal domains. CAP-23 was encoded by an mRNA of approximately 1.5 kb, whose distribution during chick development resembled that of the corresponding protein. Southern blot analysis revealed the presence of a single main hybridizing species in the chick genome. The distribution of CAP-23 during development was analyzed with Western blots and by immunofluorescence on tissue sections. In cultured cells the protein appeared to be distributed in a regular spotted pattern below the entire cell surface. In early chick embryos (E2), CAP-23 was present in most if not all cells. The protein then became progressively restricted to only some developing tissues and to only certain cells in these tissues. In most tissues CAP-23 levels fell below detection limits between E15 and E19. Highest levels of the protein were found in the nervous system, where CAP-23 levels peaked around E18, and where elevated levels were still detectable at birth.
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影响因子：
64.5
作者：
BASI, GS;JACOBSON, RD;SKENE, JHP
通讯作者：
SKENE, JHP
DOI：
10.1083/jcb.96.1.230
发表时间：
1983-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hubbard AL;Ma A
通讯作者：
Ma A
影响因子：
64.8
作者：
GOSLIN, K;SCHREYER, DJ;BANKER, G
通讯作者：
BANKER, G
DOI：
10.1073/pnas.83.10.3537
发表时间：
1986-05-01
影响因子：
11.1
作者：
MEIRI, KF;PFENNINGER, KH;WILLARD, MB
通讯作者：
WILLARD, MB
影响因子：
11.4
作者：
MYERS, MW;LAZZARINI, RA;NELSON, DL
通讯作者：
NELSON, DL