A new cluster of rhabdovirus detected in field-caught sand flies (Diptera: Psychodidae: Phlebotominae) collected from southern Thailand.
A new cluster of rhabdovirus detected in field-caught sand flies (Diptera: Psychodidae: Phlebotominae) collected from southern Thailand.
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DOI：
10.1186/s13071-021-05047-z
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发表时间：
2021-11-08
影响因子：
3.2
通讯作者：
Siriyasatien P
中科院分区：
文献类型：
--
作者：
Phumee A;Wacharapluesadee S;Petcharat S;Siriyasatien P
由于气候变化，白纹伊蚊的分布正在迅速变化。这一问题对沙蝇传播的疾病，特别是与沙蝇相关的病毒的流行病学有影响。在泰国，很少有关于沙蝇相关病毒的研究。因此，本研究旨在对泰国南部采集的沙蝇标本中属于环状病毒、细小病毒、黄病毒属和横纹病毒科的致病RNA病毒群进行分子调查。在泰国南部Trang和Songkhla省的两个地点采集了沙蝇，并对单个沙蝇样本进行了物种鉴定和病毒检测。对环状病毒、细小病毒、黄病毒属和横纹病毒科进行了分子鉴定，阳性标本通过克隆测序、细胞培养接种和系统发育分析进行鉴定。这项研究的结果是基于对331只雌性沙蝇的分析得出的。这项分子研究揭示了在白纹伊蚊体内存在横纹病毒科病毒的证据(3/331，0.9%)。这些发现证明了一个新的弹状病毒簇，它与桑黄杆菌SigmaVirus BDSV.abc5株和昆虫特异性弹状病毒科的谱系密切相关。此外，贝叶斯树表明，这一群体的共同祖先是新城疫病毒支系。据推测，该病毒可能在进化过程中更换了宿主。然而，用RT-PCR检测环状病毒、白喉病毒和黄病毒属的特异性引物在采集的沙蝇中均为阴性。关于泰国弹状病毒科的遗传多样性和生态方面的知识有限。这是关于横纹病毒科在泰国帕帕塔西棕榈树上传播的第一个数据。我们发现了一群新的弹状病毒，与新的B.dorsalis和SigmaVirus很接近。有可能这个家族还有很大的多样性有待发现，对新的弹状病毒进行更广泛的调查可能会发现来自广泛不同宿主类群的病毒，并拓宽我们对弹状病毒科之间关系的理解。
The distribution of phlebotomine sand flies is changing rapidly due to climate change. This issue has implications for the epidemiology of sand fly-borne diseases, especially sand fly-associated viruses. Few studies concerning sand fly-associated viruses have been conducted in Thailand. Therefore, this study aimed to perform a molecular survey of groups of pathogenic RNA viruses belonging to the Orbivirus, Phlebovirus, and Flavivirus genera and family Rhabdoviridae in sand fly samples collected from southern Thailand. Sand flies were collected at two locations in Trang and Songkhla provinces of southern Thailand, and individual sand fly samples were processed for species identification and virus detection. The Orbivirus, Phlebovirus, and Flavivirus genera and family Rhabdoviridae molecular determination was performed by RT-PCR, and positive samples were identified by cloning and sequencing, cell culture inoculation, and phylogenetic analysis. The results presented in this study were based on the analysis of a total of 331 female sand flies. This molecular study revealed evidence of Rhabdoviridae family virus presence in Phlebotomus papatasi (3/331, 0.9%). The findings demonstrated a new cluster of rhabdovirus that was closely related to Bactrocera dorsalis sigmavirus strain BDSV.abc5 and the lineages of insect-specific Rhabdoviridae. In addition, the Bayesian tree suggested that the common ancestor of this group was the dimarhabdovirus clade. It was assumed that the virus may have switched hosts during its evolution. However, the detection of Orbivirus, Phlebovirus, and Flavivirus genera using specific primers for RT-PCR was negative in the collected sand flies. There is limited knowledge on the genetic diversity and ecology of Rhabdoviridae in Thailand. This is the first data regarding the circulation of Rhabdoviridae in Ph. papatasi from Thailand. We found a new cluster of rhabdoviruses that was close to the new B. dorsalis sigmavirus. It is possible that there is a great deal of diversity in this family yet to be discovered, and a more extensive survey for new rhabdoviruses may uncover viruses from a wide diversity of host taxa and broaden our understanding of the relationships among the Rhabdoviridae.
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影响因子：
4.8
作者：
Lelli D;Prosperi A;Moreno A;Chiapponi C;Gibellini AM;De Benedictis P;Leopardi S;Sozzi E;Lavazza A
通讯作者：
Lavazza A
影响因子：
3.3
作者：
Longdon B;Wilfert L;Obbard DJ;Jiggins FM
通讯作者：
Jiggins FM
DOI：
10.1098/rspb.2008.0284
发表时间：
2008-07-22
影响因子：
4.7
作者：
Davies, T. Jonathan;Pedersen, Amy B.
通讯作者：
Pedersen, Amy B.
DOI：
10.1098/rstb.2019.0296
发表时间：
2019-09-30
影响因子：
6.3
作者：
Guth, Sarah;Visher, Elisa;Brook, Cara E.
通讯作者：
Brook, Cara E.
影响因子：
2.7
作者：
Afonso CL;Amarasinghe GK;Bányai K;Bào Y;Basler CF;Bavari S;Bejerman N;Blasdell KR;Briand FX;Briese T;Bukreyev A;Calisher CH;Chandran K;Chéng J;Clawson AN;Collins PL;Dietzgen RG;Dolnik O;Domier LL;Dürrwald R;Dye JM;Easton AJ;Ebihara H;Farkas SL;Freitas-Astúa J;Formenty P;Fouchier RA;Fù Y;Ghedin E;Goodin MM;Hewson R;Horie M;Hyndman TH;Jiāng D;Kitajima EW;Kobinger GP;Kondo H;Kurath G;Lamb RA;Lenardon S;Leroy EM;Li CX;Lin XD;Liú L;Longdon B;Marton S;Maisner A;Mühlberger E;Netesov SV;Nowotny N;Patterson JL;Payne SL;Paweska JT;Randall RE;Rima BK;Rota P;Rubbenstroth D;Schwemmle M;Shi M;Smither SJ;Stenglein MD;Stone DM;Takada A;Terregino C;Tesh RB;Tian JH;Tomonaga K;Tordo N;Towner JS;Vasilakis N;Verbeek M;Volchkov VE;Wahl-Jensen V;Walsh JA;Walker PJ;Wang D;Wang LF;Wetzel T;Whitfield AE;Xiè JT;Yuen KY;Zhang YZ;Kuhn JH
通讯作者：
Kuhn JH