Isolation of a novel Rhabdovirus from an insectivorous bat (Pipistrellus kuhlii) in Italy.
Isolation of a novel Rhabdovirus from an insectivorous bat (Pipistrellus kuhlii) in Italy.
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DOI：
10.1186/s12985-018-0949-z
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发表时间：
2018-02-17
期刊：
影响因子：
4.8
通讯作者：
Lavazza A
中科院分区：
文献类型：
--
作者：
Lelli D;Prosperi A;Moreno A;Chiapponi C;Gibellini AM;De Benedictis P;Leopardi S;Sozzi E;Lavazza A
弹状病毒科是最具生态多样性的RNA病毒家族之一，可感染多种脊椎动物和无脊椎动物。在哺乳动物中，蝙蝠被认为携带着更高比例的未知或新出现的具有人畜共患病潜力的病毒。在此，我们报告了在意大利北部实施的蝙蝠病毒学调查框架内发现的一种新的横纹状病毒。对635只蝙蝠标本进行了病毒分离鉴定，方法包括细胞培养、负染电子显微镜和PCRS。通常对显示细胞病变效应的细胞培养上清液进行NGS，或者在诊断方案中至少有一种PCR结果为阳性的情况下进行NGS。从库氏拟线虫的不同器官中分离到一株横纹夜蛾病毒。用电子显微镜和NGS测序进行病毒鉴定。全基因组大小为11,774个，包括5个基因，编码典型的横纹肌肉病毒结构蛋白，以及一个额外的转录单位(U1)，编码一个假想的小蛋白(157AA)(3‘-N-P-M-G-U1-L-5’)。基因组组织和系统发育分析表明，新病毒命名为Vaprio病毒(VAPV)，属于最近建立的LedanteVirus(B亚群)，与其已知近亲Le Dantec Virus(LDV)(人类，1965年塞内加尔)有很大的差异。建立了一种特异的RT-PCR方法，扩增了编码L蛋白的ORF6基因的350bp片段，并用该方法对来自同一地区和时期的76只蝙蝠进行了回顾性检测，又发现了2只VAPV阳性蝙蝠。VAPV是翼手目横纹夜蛾病毒的一个新分离株。基因组组织和系统发育分析表明，VAPV应被认为是LedanteVirus属中的一个新物种，应对其病毒生态学和疾病相关性进行研究。
Rhabdoviridae is one of the most ecologically diverse families of RNA viruses which can infect a wide range of vertebrates and invertebrates. Bats, among mammals, are pointed to harbor a significantly higher proportion of unknown or emerging viruses with zoonotic potential. Herein, we report the isolation of a novel rhabdovirus, detected in the framework of a virological survey on bats implemented in North Italy. Virus isolation and identification were performed on samples of 635 bats by using cell cultures, negative staining electron microscopy and PCRs for different viruses. NGS was commonly performed on cell culture supernatants showing cytopathic effect or in case of samples resulted positive by at least one of the PCRs included in the diagnostic protocol. A rhabdovirus was isolated from different organs of a Pipistrellus kuhlii. Virus identification was obtained by electron microscopy and NGS sequencing. The complete genome size was 11,774 nt comprised 5 genes, encoding the canonical rhabdovirus structural proteins, and an additional transcriptional unit (U1) encoding a hypothetical small protein (157aa) (3’-N-P-M-G-U1-L-5′). The genome organization and phylogenetic analysis suggest that the new virus, named Vaprio virus (VAPV), belongs to the recently established genus Ledantevirus (subgroup B) and it is highly divergent to its closest known relative, Le Dantec virus (LDV) (human, 1965 Senegal). A specific RT-PCR amplifying a 350 bp fragment of the ORF 6 gene, encoding for L protein, was developed and used to test retrospectively a subset of 76 bats coming from the same area and period, revealing two more VAPV positive bats. VAPV is a novel isolate of chiropteran rhabdovirus. Genome organization and phylogenetic analyses demonstrated that VAPV should be considered a novel species within the genus Ledantevirus for which viral ecology and disease associations should be investigated.
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DOI：
10.3390/v5112679
发表时间：
2013-10-31
期刊：
Viruses
影响因子：
--
作者：
Lelli D;Papetti A;Sabelli C;Rosti E;Moreno A;Boniotti MB
通讯作者：
Boniotti MB
影响因子：
4.8
作者：
Fischer, Melina;Freuling, Conrad M.;Hoffmann, Bernd
通讯作者：
Hoffmann, Bernd
影响因子：
3.8
作者：
Aznar-Lopez, Carolina;Vazquez-Moron, Sonia;Echevarria, Juan
通讯作者：
Echevarria, Juan
影响因子：
3.8
作者：
Kading, Rebekah C.;Gilbert, Amy T.;Miller, Barry R.
通讯作者：
Miller, Barry R.
影响因子：
10.7
作者：
Nguyen LT;Schmidt HA;von Haeseler A;Minh BQ
通讯作者：
Minh BQ