Object Recognition Memory: Distinct Yet Complementary Roles of the Mouse CA1 and Perirhinal Cortex.
Object Recognition Memory: Distinct Yet Complementary Roles of the Mouse CA1 and Perirhinal Cortex.
复制标题
DOI：
10.3389/fnmol.2020.527543
复制
发表时间：
2020
影响因子：
4.8
通讯作者：
Stackman RW Jr
中科院分区：
文献类型：
--
作者：
Cinalli DA Jr;Cohen SJ;Guthrie K;Stackman RW Jr
虽然海马体对空间记忆的重要贡献已经得到证实，但物体识别记忆传统上被认为是由海马体周围皮质(PRH)完成的。然而，一些研究的结果表明，在特定的程序条件下，根据自发对象识别(SOR)任务的测量，海马区的暂时性或永久性损害影响对象记忆过程。根据记忆强度的不同，PRH和海马体被认为对物体识别记忆有显著贡献。允许小鼠在任务的编码阶段(即样本会话)更多或更少地探索新对象，分别产生更强或更弱的对象记忆。目前的研究采用暂时性局部失活和免疫组织化学的方法来确定PRH和CA1区神经元活动对强弱物体记忆的不同贡献。在SOR样本会话后立即暂时失活CA1损害了强对象记忆，但保留了弱对象记忆；而临时失活样本后PRH损害了弱对象记忆，但保留了强对象记忆。此外，情景记忆的巩固需要mRNA转录和从头蛋白合成，即刻早期基因(IEGs)的激活模式，如c-Fos和Arc，与行为触发的神经元激活和突触可塑性有关。免疫组织化学方法检测PRH和CA1神经元中c-Fos和Arc蛋白的表达，定量聚合酶链式反应分析SOR不同阶段后Arc基因的表达，进一步支持强目标记忆依赖于CA1神经元的活动，而弱目标记忆依赖于PRH神经元的活动。综上所述，这些结果支持了在不同条件下PRH和CA1都是客体记忆所必需的观点。具体地说，我们的结果与一个模型是一致的，该模型认为，当鼠标开始探索一个新的对象时，关于它的信息在PRH中积累，并且对该对象的弱记忆被编码。如果对象探索持续超过某个阈值，则对对象探索事件的强记忆被编码；其合并依赖于CA1。这些数据通过证明CA1和PRH神经元在啮齿动物物体记忆中的功能和互补作用，有助于调和文献中的分歧。
While the essential contribution of the hippocampus to spatial memory is well established, object recognition memory has been traditionally attributed to the perirhinal cortex (PRh). However, the results of several studies indicate that under specific procedural conditions, temporary or permanent lesions of the hippocampus affect object memory processes as measured in the Spontaneous Object Recognition (SOR) task. The PRh and hippocampus are considered to contribute distinctly to object recognition memory based on memory strength. Allowing mice more, or less, exploration of novel objects during the encoding phase of the task (i.e., sample session), yields stronger, or weaker, object memory, respectively. The current studies employed temporary local inactivation and immunohistochemistry to determine the differential contributions of neuronal activity in PRh and the CA1 region of the hippocampus to strong and weak object memory. Temporary inactivation of the CA1 immediately after the SOR sample session impaired strong object memory but spared weak object memory; while temporary inactivation of PRh post-sample impaired weak object memory but spared strong object memory. Furthermore, mRNA transcription and de novo protein synthesis are required for the consolidation of episodic memory, and activation patterns of immediate early genes (IEGs), such as c-Fos and Arc, are linked to behaviorally triggered neuronal activation and synaptic plasticity. Analyses of c-Fos and Arc protein expression in PRh and CA1 neurons by immunohistochemistry, and of Arc mRNA by qPCR after distinct stages of SOR, provide additional support that strong object memory is dependent on CA1 neuronal activity, while weak object memory is dependent on PRh neuronal activity. Taken together, the results support the view that both PRh and CA1 are required for object memory under distinct conditions. Specifically, our results are consistent with a model that as the mouse begins to explore a novel object, information about it accumulates within PRh, and a weak memory of the object is encoded. If object exploration continues beyond some threshold, strong memory for the event of object exploration is encoded; the consolidation of which is CA1-dependent. These data serve to reconcile the dissension in the literature by demonstrating functional and complementary roles for CA1 and PRh neurons in rodent object memory.
登录
查看更多内容
影响因子：
64.8
作者：
Bunsey, M;Eichenbaum, H
通讯作者：
Eichenbaum, H
影响因子：
3.1
作者：
Bernstein, Hannah L.;Lu, Yi-Ling;Scharfman, Helen E.
通讯作者：
Scharfman, Helen E.
影响因子：
2.7
作者：
Cohen SJ;Stackman RW Jr
通讯作者：
Stackman RW Jr
影响因子：
8.2
作者：
Brown, M. W.;Banks, P. J.
通讯作者：
Banks, P. J.
影响因子：
2.5
作者：
de Lima, Maria Noemia;Luft, Tatiana;Schroder, Nadja
通讯作者：
Schroder, Nadja