Engineering self-assembled neomenisci through combination of matrix augmentation and directional remodeling.
Engineering self-assembled neomenisci through combination of matrix augmentation and directional remodeling.
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DOI：
10.1016/j.actbio.2020.04.019
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发表时间：
2020-06
影响因子：
9.7
通讯作者：
Athanasiou KA
中科院分区：
文献类型：
--
作者：
Gonzalez-Leon EA;Bielajew BJ;Hu JC;Athanasiou KA
膝关节半月板损伤很常见，在美国和欧洲每年有超过100万例手术。由于这种纤维软骨组织几乎是无血管的，而且它本身就缺乏愈合，组织工程学已经被提出作为半月板修复和替换的解决方案。这项研究描述了一种使用生物活性刺激来提高细胞外基质含量和新月板的组织以增强其机械性能的方法。在溶血磷脂酸(LPA)的基础上，用转化生长因子-β1、软骨素酶ABC和赖氨酰氧化酶样2(统称为TCL)处理自组装纤维软骨。TCL+LPA治疗协同改善了环向拉伸硬度和强度，显著提高了单位干重的胶原和吡啶酚交联物含量，并使新薄膜层的拉伸各向异性(周向/径向)值接近4。本研究利用多种生物活性刺激的组合用于组织工程研究，为将新薄膜层用作功能修复和替代组织提供了一条有前景的途径。
Knee meniscus injury is frequent, resulting in over 1 million surgeries annually in the United States and Europe. Because of the near-avascularity of this fibrocartilaginous tissue and its intrinsic lack of healing, tissue engineering has been proposed as a solution for meniscus repair and replacement. This study describes an approach employing bioactive stimuli to enhance both extracellular matrix content and organization of neomenisci toward augmenting their mechanical properties. Self-assembled fibrocartilages were treated with TGF-β1, chondroitinase ABC, and lysyl oxidase-like 2 (collectively termed TCL) in addition to lysophosphatidic acid (LPA). TCL+LPA treatment synergistically improved circumferential tensile stiffness and strength, significantly enhanced collagen and pyridinoline crosslink content per dry weight, and achieved tensile anisotropy (circumferential/radial) values of neomenisci close to 4. This study utilizes a combination of bioactive stimuli for use in tissue engineering studies, providing a promising path toward deploying these neomenisci as functional repair and replacement tissues.
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影响因子：
14
作者：
Gunja, Najmuddin J.;Uthamanthil, Rajesh K.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.
影响因子：
--
作者：
Baek J;Sovani S;Glembotski NE;Du J;Jin S;Grogan SP;D'Lima DD
通讯作者：
D'Lima DD
影响因子：
3
作者：
Almarza, AJ;Athanasiou, KA
通讯作者：
Athanasiou, KA
影响因子：
14
作者：
Huey, Daniel J.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.
影响因子：
3.8
作者：
Detamore, MS;Athanasiou, KA
通讯作者：
Athanasiou, KA