Hypochlorous acid-generating electrochemical scaffold eliminates Candida albicans biofilms.
Hypochlorous acid-generating electrochemical scaffold eliminates Candida albicans biofilms.
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DOI：
10.1111/jam.14656
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发表时间：
2020-10
影响因子：
4
通讯作者：
Beyenal H
中科院分区：
文献类型：
--
作者：
Zmuda HM;Mohamed A;Raval YS;Call DR;Schuetz AN;Patel R;Beyenal H
涉及白色念珠菌的伤口感染可能具有挑战性，因为真菌能够穿透伤口组织并形成生物膜。本研究的目的是评估次氯酸（HOCl）生成电化学支架（e-支架）对C。体外和在猪皮肤外植体（离体）上的白色念珠菌生物膜。C.白色念珠菌生物膜在丙烯酸底六孔板（体外）或从猪耳切除的皮肤组织（离体）上生长，并使用极化的e-支架在生物膜表面附近产生连续供应的低浓度HOCl。C.与对照生物膜（5.28 ± 0.034 log 10 CFU cm−2; P < 0.0001）不同，在体外生长的白色念珠菌生物膜在暴露于e-支架24小时内减少至不可检测的量。C.与对照外植体生物膜（4.29 ± 0.057 log 10 CFU cm-2; P < 0.0001）不同，在猪皮肤外植体上生长的白色念珠菌生物膜也在24小时内减少至不可检测的量。与暴露于非极化的e-支架（不产生HOCl）的外植体相比，暴露于连续产生HOCl的e-支架24小时的未感染的猪真皮外植体中活哺乳动物细胞的数量减少（35.6 ± 6.4%）（P < 0.05）。我们的HOCl生成e-支架是治疗C.白色念珠菌生物膜。伤口感染由C.白色念珠菌由于在伤口床中存在生物膜而难以治疗。我们的HOCl生产e-支架提供了一种有前途的新方法来治疗由C.白色念珠菌
Wound infections involving Candida albicans can be challenging to treat because of the fungus’ ability to penetrate wound tissue and form biofilms. The goal of this study was to assess the activity of a hypochlorous acid (HOCl)-generating electrochemical scaffold (e-scaffold) against C. albicans biofilms in vitro and on porcine dermal explants (ex vivo). C. albicans biofilms were grown either on acrylic-bottom six-well plates (in vitro) or on skin tissue excised from porcine ears (ex vivo), and the polarized e-scaffold was used to generate a continuous supply of low concentration HOCl near biofilm surfaces. C. albicans biofilms grown in vitro were reduced to undetectable amounts within 24 h of e-scaffold exposure, unlike control biofilms (5.28 ± 0.034 log10 CFU cm−2; P < 0.0001). C. albicans biofilms grown on porcine dermal explants were also reduced to undetectable amounts in 24 h, unlike control explant biofilms (4.29 ± 0.057 log10 CFU cm−2; P < 0.0001). There was a decrease in the number of viable mammalian cells (35.6 ± 6.4%) in uninfected porcine dermal explants exposed to continuous HOCl-generating e-scaffolds for 24 h compared to explants exposed to nonpolarized e-scaffolds (not generating HOCl) (P < 0.05). Our HOCl-generating e-scaffold is a potential antifungal-free strategy to treat C. albicans biofilms in chronic wounds. Wound infections caused by C. albicans are difficult to treat due to presence of biofilms in wound beds. Our HOCl producing e-scaffold provides a promising novel approach to treat wound infections caused by C. albicans.
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影响因子：
4.8
作者：
BOURGUIGNON, GJ;BOURGUIGNON, LYW
通讯作者：
BOURGUIGNON, LYW
影响因子：
4.9
作者：
Bachmann, SP;VandeWalle, K;López-Ribot, JL
通讯作者：
López-Ribot, JL
DOI：
10.1111/j.1365-2672.1993.tb04345.x
发表时间：
1993-01-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
BROWN, MRW;GILBERT, P
通讯作者：
GILBERT, P
影响因子：
4.9
作者：
Harriott, Melphine M.;Noverr, Mairi C.
通讯作者：
Noverr, Mairi C.
影响因子：
1.9
作者：
Dowd, S. E.;Hanson, J. Delton;Jones, C. E.
通讯作者：
Jones, C. E.