Effects of Growth Stage on the Characterization of Enterotoxin A-Producing Staphylococcus aureus-Derived Membrane Vesicles.
Effects of Growth Stage on the Characterization of Enterotoxin A-Producing Staphylococcus aureus-Derived Membrane Vesicles.
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生长阶段对产肠毒素A金黄色葡萄球菌衍生膜囊泡特性的影响。
DOI：
10.3390/microorganisms10030574
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发表时间：
2022-03-06
期刊：
影响因子：
4.5
通讯作者：
Masuda S
中科院分区：
文献类型：
--
作者：
Yamanashi Y;Shimamura Y;Sasahara H;Komuro M;Sasaki K;Morimitsu Y;Masuda S
毒力因子，如金黄色葡萄球菌肠毒素A（SEA），包含在金黄色葡萄球菌细胞膜中的膜囊泡（MV）内。本研究探讨了生长阶段对大豆叶片中各组分的质和量的变化的影响。检测金黄色葡萄球菌产SEA菌株。S.结果表明，金葡菌在各生长阶段的基因表达量均有所下降，其中酚可溶性调节蛋白（PSM）基因在8 h后表达量达到最大值，细胞膜相关基因在6 h后表达量下降。基于这些基因表达模式，在6、17和24 h制备MV。MV的颗粒尺寸不随生长阶段而变化。培养17小时后制备的MV在23 °C下储存时保持其粒径。培养上清中SEA含量与MV无相关性。双功能自溶素是一种参与细胞壁生物合成/降解的蛋白质，在17 h时在MV中增加。MVs诱导的人成体低钙高温（HaCaT）细胞中炎症相关基因的表达模式在各个生长阶段不同。S.金黄色葡萄球菌衍生的MV是选择性的，取决于生长阶段，并可能在毒性中发挥重要作用。
Virulence factors, such as staphylococcal enterotoxin A (SEA), are contained within membrane vesicles (MVs) in the cell membrane of Staphylococcus aureus. In this study, the effects of the growth stage on quantitative and qualitative changes in the components contained in the MVs of S. aureus SEA-producing strains were examined. Changes in the expression levels of S. aureus genes were examined at each growth stage; phenol-soluble modulin (PSM) gene reached a maximum after 8 h, and the expression of cell membrane-related genes was decreased after 6 h. Based on these gene expression patterns, MVs were prepared at 6, 17, and 24 h. The particle size of MVs did not change depending on the growth stage. MVs prepared after culture for 17 h maintained their particle size when stored at 23 °C. The amount of SEA in the culture supernatant and MVs were not correlated. Bifunctional autolysin, a protein involved in cell wall biosynthesis/degradation, was increased in MVs at 17 h. The expression pattern of inflammation-related genes in human adult low calcium high temperature (HaCaT) cells induced by MVs was different for each growth stage. The inclusion components of S. aureus-derived MVs are selective, depend on the stage of growth, and may play an important role in toxicity.
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DOI：
10.1038/nrmicro3480
发表时间：
2015-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Brown L;Wolf JM;Prados-Rosales R;Casadevall A
通讯作者：
Casadevall A
影响因子：
14.8
作者：
Carnes EC;Lopez DM;Donegan NP;Cheung A;Gresham H;Timmins GS;Brinker CJ
通讯作者：
Brinker CJ
DOI：
10.1016/j.bbalip.2012.08.007
发表时间：
2013-03
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Dowhan W
通讯作者：
Dowhan W
影响因子：
4.8
作者：
Koprivnjak, T;Peschel, A;Weiss, JP
通讯作者：
Weiss, JP
影响因子：
4.3
作者：
Han, Eun Hee;Park, Lin Hee;Jeong, Hye Gwang
通讯作者：
Jeong, Hye Gwang