Comparative proteomic and transcriptomic analysis reveals high pH–induced expression signatures of Chinese shrimp Fenneropenaeus chinensis
Comparative proteomic and transcriptomic analysis reveals high pH–induced expression signatures of Chinese shrimp Fenneropenaeus chinensis
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比较蛋白质组学和转录组学分析揭示了中国明对虾的高 pH 诱导表达特征
DOI：
10.1007/s10142-021-00779-8
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发表时间：
2021-02
影响因子：
2.9
通讯作者：
Yuying He
中科院分区：
文献类型：
--
作者：
Zhaoxia Li;Xiaoqi Tang;Jian Li;Yuying He
pH值对许多水生动物的分布、生长、行为和生理有很大影响。使用 iTRAQ 蛋白质组分析成功地实现了正常和高 pH 胁迫样品之间的蛋白质组比较，以筛选关键响应蛋白质和途径。高 pH 胁迫后，鉴定出 124 个上调蛋白和 41 个下调蛋白。柠檬酸合酶、谷胱甘肽 S-转移酶、谷胱甘肽过氧化物酶和细胞色素 C 氧化酶等蛋白质的较高表达水平与氧化应激和线粒体功能障碍有关。葡萄糖调节蛋白78的上调表明内质网应激是由高pH应激诱导的。高pH暴露后，V型H+-ATP酶、Na+、K+-ATP酶、14-3-3蛋白以及ATP结合盒跨膜转运蛋白的表达显着上调，这表明它们在响应高pH胁迫中发挥重要作用。高pH组中参与蛋白质糖基化、氧化戊糖磷酸途径、蛋白质输出和谷胱甘肽代谢的蛋白质丰度比对照组丰富。
pH has a great impact on the distribution, growth, behavior, and physiology in many aquatic animals. The comparison of proteomics between normal and high pH stress samples was successfully achieved using iTRAQ proteomic analysis to screen key response proteins and pathways. After high pH stress, 124 upregulated and 41 downregulated proteins were identified. The higher expression levels of proteins like citrate synthase, glutathione S-transferase, glutathione peroxidase, and cytochrome c oxidase are associated with oxidative stress and mitochondrial dysfunction. The upregulation of glucose-regulated protein 78 indicated that the endoplasmic reticulum stress is induced by high pH stress. There were significant upregulation expressions of V-type H+-ATPase, Na+, K+-ATPase, 14-3-3 protein, as well as ATP-binding cassette transmembrane transporters after high pH exposure, which indicating their important roles in response to high pH stress. The abundance of proteins involved in protein glycosylation, oxidative pentose phosphate pathway, protein export, and glutathione metabolism were found enriched in high pH group than in control group.
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影响因子：
5.6
作者：
Mustafa G;Komatsu S
通讯作者：
Komatsu S
影响因子：
--
作者：
Andersen V;Ernst A;Christensen J;Østergaard M;Jacobsen BA;Tjønneland A;Krarup HB;Vogel U
通讯作者：
Vogel U
影响因子：
4.7
作者：
Lin, Tingting;Lai, Qifang;Wang, Hui
通讯作者：
Wang, Hui
影响因子：
4.5
作者：
L. Pan;Linjuan Zhang;Hong-yu Liu
通讯作者：
L. Pan;Linjuan Zhang;Hong-yu Liu
影响因子：
2.6
作者：
Yongeun Kim;H. Mo;Jino Son;Yun-Sik Lee;Sung-Eun Lee;Kijong Cho
通讯作者：
Yongeun Kim;H. Mo;Jino Son;Yun-Sik Lee;Sung-Eun Lee;Kijong Cho