Comparative sequence analyses reveal sites of ancestral chromosomal fusions in the Indian muntjac genome.
Comparative sequence analyses reveal sites of ancestral chromosomal fusions in the Indian muntjac genome.
复制标题
DOI：
10.1186/gb-2008-9-10-r155
复制
发表时间：
2008-10-28
期刊：
影响因子：
12.3
通讯作者：
Green ED
中科院分区：
文献类型：
--
作者：
Tsipouri V;Schueler MG;Hu S;NISC Comparative Sequencing Program;Dutra A;Pak E;Riethman H;Green ED
使用比较作图和测序来表征印度麂基因组中祖先染色体融合的位点。印度麂（Muntiacus muntjak vaginalis）具有极端的哺乳动物核型，雌性和雄性分别只有 6 条和 7 条染色体。中国麂（Muntiacus reevesi）具有更典型的哺乳动物核型，两性都有46条染色体。尽管存在这种差异，但这两个麂物种在形态上相似，甚至可以杂交产生可存活（尽管不育）的后代。先前的研究表明，一系列涉及端粒和/或卫星重复的端粒染色体融合事件导致了现存的印度麂核型。我们使用比较作图和测序方法来表征印度麂基因组中祖先染色体融合的位点。具体来说，我们用端粒重复特异性探针筛选了印度麂细菌人工染色体文库。通过荧光原位杂交发现的分离克隆，映射到印度麂染色体上的间隙区域，进行了进一步的表征，然后对其中的一个子集进行了鸟枪测序。随后，我们分离并测序了从其中一些初始克隆末端延伸的重叠克隆；我们还从分离的中国麂克隆中生成了直系同源序列。已对生成的印度麂序列进行了端粒重复和卫星重复的并置以及相对于其他哺乳动物基因组（包括中国麂）的同线性关系的分析。生成的序列数据和比较分析为印度麂基因组中的七个祖先染色体融合位点提供了详细的基因组背景，这进一步支持了导致麂物种之间异常核型差异的事件的端粒融合模型。
Comparative mapping and sequencing was used to characterize the sites of ancestral chromosomal fusions in the Indian muntjac genome. Indian muntjac (Muntiacus muntjak vaginalis) has an extreme mammalian karyotype, with only six and seven chromosomes in the female and male, respectively. Chinese muntjac (Muntiacus reevesi) has a more typical mammalian karyotype, with 46 chromosomes in both sexes. Despite this disparity, the two muntjac species are morphologically similar and can even interbreed to produce viable (albeit sterile) offspring. Previous studies have suggested that a series of telocentric chromosome fusion events involving telomeric and/or satellite repeats led to the extant Indian muntjac karyotype. We used a comparative mapping and sequencing approach to characterize the sites of ancestral chromosomal fusions in the Indian muntjac genome. Specifically, we screened an Indian muntjac bacterial artificial-chromosome library with a telomere repeat-specific probe. Isolated clones found by fluorescence in situ hybridization to map to interstitial regions on Indian muntjac chromosomes were further characterized, with a subset then subjected to shotgun sequencing. Subsequently, we isolated and sequenced overlapping clones extending from the ends of some of these initial clones; we also generated orthologous sequence from isolated Chinese muntjac clones. The generated Indian muntjac sequence has been analyzed for the juxtaposition of telomeric and satellite repeats and for synteny relationships relative to other mammalian genomes, including the Chinese muntjac. The generated sequence data and comparative analyses provide a detailed genomic context for seven ancestral chromosome fusion sites in the Indian muntjac genome, which further supports the telocentric fusion model for the events leading to the unusual karyotypic differences among muntjac species.
登录
查看更多内容
影响因子：
1.7
作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
Walichiewicz, J
影响因子：
1.6
作者：
BOGENBERGER, J;SCHNELL, H;FITTLER, F
通讯作者：
FITTLER, F
DOI：
10.1159/000130497
发表时间：
1975-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
HSU, TC;PATHAK, S;CHEN, TR
通讯作者：
CHEN, TR
影响因子：
2.6
作者：
Fronicke, L;Scherthan, H
通讯作者：
Scherthan, H
影响因子：
2.6
作者：
Huang, L.;Wang, J.;Yang, F.
通讯作者：
Yang, F.