In vivo monitoring of structural and mechanical changes of tissue scaffolds by multi-modality imaging.
In vivo monitoring of structural and mechanical changes of tissue scaffolds by multi-modality imaging.
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DOI：
10.1016/j.biomaterials.2014.05.088
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发表时间：
2014-09
期刊：
影响因子：
14
通讯作者：
Kim, Kang
中科院分区：
文献类型：
--
作者：
Park, Dae Woo;Ye, Sang-Ho;Jiang, Hong Bin;Dutta, Debaditya;Nonaka, Kazuhiro;Wagner, William R.;Kim, Kang
关键词：
植入可降解的组织支架以在愈合过程发生时起到机械作用，并且随着支架降解，患者承担生理负荷。在此期间的机械失效可能是不可预测的，因为在体内和非侵入性地监测植入部位的结构降解和机械强度变化并不容易实现。为了满足这一需求，使用超声剪切波成像（USWI）和光声成像（派）进行体内机械和结构评估的多模态方法被证明与可降解的聚（酯氨基甲酸酯）脲（PEUU）和聚对二氧环己酮（PDO）支架。采用湿法静电纺丝法制备纤维支架，吲哚菁绿色（ICG）染色，与派形成光学对比，植入36只大鼠腹壁。每月使用USWI和派监测支架，并在0、4、8和12周时取出支架进行力学和组织学评估。通过USWI获得的体内结构的剪切模量变化与通过压缩测量获得的离体结构的平均杨氏模量变化相关。PEUU和PDO支架表现出明显不同的降解速率和平均派信号强度。派信号强度的分布也与外植体组织学中观察到的剩余支架很好地对应。使用小动物腹壁修复模型的这一证据表明，USWI和派的多模态成像可以允许组织工程师在各种应用中无创地评估体内组织结构的并发机械刚度和结构变化。
Degradable tissue scaffolds are implanted to serve a mechanical role while healing processes occur and putatively assume the physiological load as the scaffold degrades. Mechanical failure during this period can be unpredictable as monitoring of structural degradation and mechanical strength changes at the implant site is not readily achieved in vivo, and non-invasively. To address this need, a multi-modality approach using ultrasound shear wave imaging (USWI) and photoacoustic imaging (PAI) for both mechanical and structural assessment in vivo was demonstrated with degradable poly(ester urethane)urea (PEUU) and polydioxanone (PDO) scaffolds. The fibrous scaffolds were fabricated with wet electrospinning, dyed with indocyanine green (ICG) for optical contrast in PAI, and implanted in the abdominal wall of 36 rats. The scaffolds were monitored monthly using USWI and PAI and were extracted at 0, 4, 8 and 12 wk for mechanical and histological assessment. The change in shear modulus of the constructs in vivo obtained by USWI correlated with the change in average Young's modulus of the constructs ex vivo obtained by compression measurements. The PEUU and PDO scaffolds exhibited distinctly different degradation rates and average PAI signal intensity. The distribution of PAI signal intensity also corresponded well to the remaining scaffolds as seen in explant histology. This evidence using a small animal abdominal wall repair model demonstrates that multi-modality imaging of USWI and PAI may allow tissue engineers to noninvasively evaluate concurrent mechanical stiffness and structural changes of tissue constructs in vivo for a variety of applications.
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影响因子：
14
作者：
Hashizume, Ryotaro;Fujimoto, Kazuro L.;Hong, Yi;Amoroso, Nicholas J.;Tobita, Kimimasa;Miki, Toshio;Keller, Bradley B.;Sacks, Michael S.;Wagner, William R.
通讯作者：
Wagner, William R.
DOI：
10.1109/58.852079
发表时间：
2000-07-01
影响因子：
3.6
作者：
Cohn, NA;Kim, BS;O'Donnell, M
通讯作者：
O'Donnell, M
DOI：
10.1002/jbm.10204
发表时间：
2002-09-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Guan, JJ;Sacks, MS;Wagner, WR
通讯作者：
Wagner, WR
影响因子：
14
作者：
Sullivan, David C.;Mirmalek-Sani, Sayed-Hadi;Yoo, James J.
通讯作者：
Yoo, James J.
影响因子：
2.9
作者：
Perni, Stefano;Pratten, Jonathan;Prokopovich, Polina
通讯作者：
Prokopovich, Polina