Functions of Armigeres subalbatus C-type lectins in innate immunity.
Functions of Armigeres subalbatus C-type lectins in innate immunity.
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DOI：
10.1016/j.ibmb.2014.06.010
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发表时间：
2014-09
影响因子：
3.8
通讯作者：
Yu, Xiao-Qiang
中科院分区：
文献类型：
--
作者：
Shi, Xiu-Zhen;Kang, Cui-Jie;Wang, Song-Jie;Zhong, Xue;Beerntsen, Brenda T.;Yu, Xiao-Qiang
C 型凝集素 (CTL) 是钙依赖性碳水化合物结合蛋白的超家族，含有至少一个碳水化合物识别结构域 (CRD)，它们存在于几乎所有后生动物中。昆虫 CTL 可能充当模式识别受体，并在先天免疫中发挥重要作用。在这项研究中，我们从丝虫线虫的天然载体——Armigeres subalbatus蚊子中选择了五个AsCTL，并进行了体外和体内研究，以阐明它们在先天免疫中的功能。 AsCTLMA15、AsCTLGA5和AsCTL15主要在血细胞中表达，AsCTL16在脂肪体中表达，而AsCTLMA11在血细胞和脂肪体中均表达，并且只有AsCTLMA11和AsCTL16在成年女性中高水平表达。体外结合试验表明，所有五种重组AsCTL都能与不同的微生物细胞壁成分结合，包括脂多糖（LPS）、脂质A、肽聚糖（PG）、脂磷壁酸（LTA）、酵母聚糖和昆布多糖（β-1,3-葡聚糖）。重组 AsCTL 还可以与多种革兰氏阴性和革兰氏阳性细菌结合，并可以凝集细菌细胞。注射双链RNA（dsRNA）可显着降低5种AsCTL mRNA的表达，并且与对照组相比，用dsRNA处理AsCTLGA5的蚊子的存活率在大肠杆菌感染后显着降低，但在藤黄微球菌感染后与对照组相比没有显着变化，这表明Ar. subalbatus AsCTLGA5 可能参与针对大肠杆菌的先天免疫。
C-type lectins (CTLs) are a superfamily of calcium-dependent carbohydrate binding proteins containing at least one carbohydrate-recognition domain (CRD) and they are present in almost all metazoans. Insect CTLs may function as pattern-recognition receptors and play important roles in innate immunity. In this study, we selected five AsCTLs from the mosquito Armigeres subalbatus, a natural vector of filarial nematodes, and performed both in vitro and in vivo studies to elucidate their functions in innate immunity. AsCTLMA15, AsCTLGA5 and AsCTL15 were mainly expressed in hemocytes, AsCTL16 was expressed in fat body, while AsCTLMA11 was expressed in both hemocytes and fat body, and only AsCTLMA11 and AsCTL16 were expressed at high levels in adult females. In vitro binding assays showed that all five recombinant AsCTLs could bind to different microbial cell wall components, including lipopolysaccharide (LPS), lipid A, peptidoglycan (PG), lipoteichoic acid (LTA), zymosan and laminarin (beta-1,3-glucan). Recombinant AsCTLs also bound to several Gram-negative and Gram-positive bacteria, and could agglutinate bacterial cells. Injection of double-stranded RNAs (dsRNAs) could significantly reduce expression of the five AsCTL mRNAs, and the survival of mosquitoes treated with dsRNA to AsCTLGA5 was significantly decreased after Escherichia coli infection, but did not change significantly after Micrococcus luteus infection compared to the control groups, suggesting that Ar. subalbatus AsCTLGA5 may participate in innate immunity against E. coli.
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影响因子：
3.7
作者：
Fang Q;Wang F;Gatehouse JA;Gatehouse AM;Chen XX;Hu C;Ye GY
通讯作者：
Ye GY
DOI：
10.2987/8756-971x(2005)21
发表时间：
2005-09-01
影响因子：
1
作者：
Grieco, JP;Achee, NL;Andre, RG
通讯作者：
Andre, RG
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影响因子：
3.9
作者：
Jack, DL;Turner, MW
通讯作者：
Turner, MW
影响因子：
4.4
作者：
Mayhew, George F.;Bartholomay, Lyric C.;Chen, Cheng-Chen
通讯作者：
Chen, Cheng-Chen
影响因子：
2.9
作者：
Ling, EJ;Yu, XQ
通讯作者：
Yu, XQ