The Microevolution and Epidemiology of Staphylococcus aureus Colonization during Atopic Eczema Disease Flare.
The Microevolution and Epidemiology of Staphylococcus aureus Colonization during Atopic Eczema Disease Flare.
复制标题
特应性湿疹病耀斑期间金黄色葡萄球菌定殖的微观进化和流行病学。
DOI：
10.1016/j.jid.2017.09.023
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Holden MTG
中科院分区：
文献类型：
--
作者：
Harkins CP;Pettigrew KA;Oravcová K;Gardner J;Hearn RMR;Rice D;Mather AE;Parkhill J;Brown SJ;Proby CM;Holden MTG
金黄色葡萄球菌是一种机会致病菌，是人类微生物群的可变组成部分。特应性湿疹（AE）的一个特征是金黄色葡萄球菌的定植，其恶化与细菌负担的增加有关。尽管如此，在AE疾病爆发期间，金黄色葡萄球菌定殖个体患者的起源和遗传多样性尚不清楚。为了研究金黄色葡萄球菌定植的微进化，我们对9名患有中度至重度AE的儿童和18名无症状鼻腔携带金黄色葡萄球菌的非特应性儿童的金黄色葡萄球菌群体进行了深度测序。在AE患者和对照组中均观察到金黄色葡萄球菌克隆群体的定植，除一人外，所有个体都携带属于单一序列类型的菌落。系统发育分析表明，疾病爆发与金黄色葡萄球菌种群的克隆扩增有关，发生时间为数周至数月。金黄色葡萄球菌定殖AE病例与对照组的遗传背景有显著差异（Fisher精确检验，P = 0.03）。对定殖金黄色葡萄球菌群体宿主内遗传异质性的研究发现了AE患者宿主内选择的证据，AE变异可能选择性地有利于细胞内持久性和治疗耐药性。
Staphylococcus aureus is an opportunistic pathogen and variable component of the human microbiota. A characteristic of atopic eczema (AE) is colonization by S. aureus, with exacerbations associated with an increased bacterial burden of the organism. Despite this, the origins and genetic diversity of S. aureus colonizing individual patients during AE disease flares is poorly understood. To examine the microevolution of S. aureus colonization, we deep sequenced S. aureus populations from nine children with moderate to severe AE and 18 non-atopic children asymptomatically carrying S. aureus nasally. Colonization by clonal S. aureus populations was observed in both AE patients and control participants, with all but one of the individuals carrying colonies belonging to a single sequence type. Phylogenetic analysis showed that disease flares were associated with the clonal expansion of the S. aureus population, occurring over a period of weeks to months. There was a significant difference in the genetic backgrounds of S. aureus colonizing AE cases versus controls (Fisher exact test, P = 0.03). Examination of intra-host genetic heterogeneity of the colonizing S. aureus populations identified evidence of within-host selection in the AE patients, with AE variants being potentially selectively advantageous for intracellular persistence and treatment resistance.
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影响因子：
15.9
作者：
Knox, Justin;Uhlemannl, Anne-Catrin;Lowy, Franklin D.
通讯作者：
Lowy, Franklin D.
DOI：
10.1159/000249493
发表时间：
1985-01-01
期刊：
DERMATOLOGICA
影响因子：
--
作者：
HAUSER, C;WUETHRICH, B;SCHOPFER, K
通讯作者：
SCHOPFER, K
影响因子：
3.1
作者：
Kim, Do-Won;Park, Jee-Young;Kim, Jungmin
通讯作者：
Kim, Jungmin
影响因子：
6.5
作者：
Lomholt, H;Andersen, KE;Kilian, M
通讯作者：
Kilian, M
DOI：
10.1038/nrmicro.2015.13
发表时间：
2016-03
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
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