Chemoattractant activity of degradation products of fetal and adult skin extracellular matrix for keratinocyte progenitor cells.
Chemoattractant activity of degradation products of fetal and adult skin extracellular matrix for keratinocyte progenitor cells.
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DOI：
10.1002/term.123
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发表时间：
2008-12
影响因子：
3.3
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Brennan, Ellen P.;Tang, Xiao-Han;Stewart-Akers, Ann M.;Gudas, Lorraine J.;Badylak, Stephen F.
由天然存在的细胞外基质（ECM）组成的生物支架已经在临床前研究和人类临床应用中用作许多组织的建设性重塑的模板。ECM诱导结构性重塑的机制尚不清楚，但ECM支架的降解产物可能在细胞募集和结构性重塑中起关键作用。本研究的目的是探讨年龄和ECM的组织是收获的ECM降解产物的人角质形成细胞干细胞和祖细胞的化学引诱活性的物种的影响。制备成人皮肤ECM、胎儿人皮肤ECM和成人猪皮肤ECM，酶促消化，通过SDS-PAGE表征，并评价对人角质形成细胞祖细胞和干细胞（HEKn）的体外化学引诱剂活性。对于HEKn，人胎儿皮肤ECM的降解产物显示出比人成人皮肤ECM降解产物更大的化学引诱物活性。猪成人皮肤ECM的降解产物显示出比人成人皮肤ECM更大的趋化活性。人胎儿皮肤ECM降解产物对HEKn表现出最强的化学引诱活性。这项研究的结果支持这样的概念，即ECM支架重塑的机制涉及通过支架降解产物招募谱系定向祖细胞，并且从中收获ECM的组织的年龄和物种都对这种化学引诱物潜力有影响。
Biologic scaffolds composed of naturally occurring extracellular matrix (ECM) have been utilized as templates for the constructive remodeling of numerous tissues in preclinical studies and human clinical applications. The mechanisms by which ECM induces constructive remodeling are not well understood, but it appears that the degradation products of ECM scaffolds may play key roles in cell recruitment and constructive remodeling. The objective of the present study was to investigate the effects of age and species of the tissue from which ECM is harvested on the chemoattractant activity of degradation products of ECM for human keratinocyte stem and progenitor cells. Adult human skin ECM, fetal human skin ECM, and adult porcine skin ECM were prepared, enzymatically digested, characterized by SDS-PAGE, and evaluated for in vitro chemoattractant activity for human keratinocyte progenitor and stem cells (HEKn). Degradation products of human fetal skin ECM showed greater chemoattractant activity than human adult skin ECM degradation products for the HEKn. Degradation products of porcine adult skin ECM showed greater chemoattractant activity than human adult skin ECM. The human fetal skin ECM degradation products showed the strongest chemoattractant activity for the HEKn. The findings of this study support the concept that the mechanism of ECM scaffold remodeling involves the recruitment of lineage-directed progenitor cells by scaffold degradation products and that both the age and species of the tissue from which the ECM is harvested have an effect upon this chemoattractant potential.
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影响因子：
2.6
作者：
Bullard, KM;Longaker, MT;Lorenz, HP
通讯作者：
Lorenz, HP
影响因子：
2.4
作者：
Badylak, S;Meurling, S;Simmons-Byrd, A
通讯作者：
Simmons-Byrd, A
影响因子：
2.4
作者：
Atala, A
通讯作者：
Atala, A
影响因子：
14
作者：
Gilbert, Thomas W.;Stewart-Akers, Ann M.;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
影响因子：
14
作者：
Gilbert, TW;Stolz, DB;Badylak, SF
通讯作者：
Badylak, SF