Ocular Blood Flow Measurements in Healthy White Subjects Using Laser Speckle Flowgraphy.
Ocular Blood Flow Measurements in Healthy White Subjects Using Laser Speckle Flowgraphy.
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使用激光斑点流程学的健康白人受试者的眼血流量测量。
DOI：
10.1371/journal.pone.0168190
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Schmetterer L
中科院分区：
文献类型：
--
作者：
Luft N;Wozniak PA;Aschinger GC;Fondi K;Bata AM;Werkmeister RM;Schmidl D;Witkowska KJ;Bolz M;Garhöfer G;Schmetterer L
评估激光散斑血流图（LSFG）在健康白色受试者样本中测量眼灌注的可行性和可靠性，并阐明所获得参数的年龄依赖性。这项横断面研究包括80名年龄在19至79岁之间的健康、不吸烟的西欧血统白色受试者的80只眼。一个商业LSFG仪器应用于测量眼血流在视神经乳头（ONH）连续三次之前和之后的药物瞳孔放大。平均模糊率（MBR），相对血流速度的测量，获得了不同区域的ONH。同时记录了由MBR脉冲波形分析得出的8个眼部灌注参数，包括井喷时间（BOT）和下降速率（FR）。符合自动图像分析质量标准的无伪影LSFG图像在无瞳孔扩张的情况下可获得93.8%，在药物瞳孔扩张的情况下可获得98.8%。MBR的测量结果显示出良好的重复性，组内相关系数≥ 0.937，几乎不受瞳孔扩张的影响。大多数脉冲波形衍生变量表现出同样高的可重复性。MBR相关血流指标显示出显著的年龄依赖性（p<0.001）。FR（r = 0.747，p<0.001）和BOT（r =-0.714，p <0.001）与年龄相关性最强。LSFG是一种可靠的方法，用于定量评估白色受试者的眼血流。我们的数据证实，LSFG衍生的变量FR和BOT可能是有用的生物标志物，与年龄相关的变化，眼灌注。
To assess the feasibility and reliability of Laser Speckle Flowgraphy (LSFG) to measure ocular perfusion in a sample of healthy white subjects and to elucidate the age-dependence of the parameters obtained. This cross-sectional study included 80 eyes of 80 healthy, non-smoking white subjects of Western European descent between 19 and 79 years of age. A commercial LSFG instrument was applied to measure ocular blood flow at the optic nerve head (ONH) three successive times before and after pharmacological pupil dilation. The mean blur rate (MBR), a measure of relative blood flow velocity, was obtained for different regions of the ONH. Eight parameters of ocular perfusion derived from the pulse-waveform analysis of MBR including blowout time (BOT) and falling rate (FR) were also recorded. Artifact-free LSFG images meeting the quality criteria for automated image analysis were obtainable in 93.8% without pupil dilation and in 98.8% with pharmacological pupil dilation. Measurements of MBR showed excellent repeatability with intraclass correlation coefficients ≥ 0.937 and were barely affected by pupil dilation. The majority of pulse-waveform derived variables exhibited equally high repeatability. MBR-related blood flow indices exhibited significant age dependence (p<0.001). FR (r = 0.747, p<0.001) and BOT (r = -0.714, p<0.001) most strongly correlated with age. LSFG represents a reliable method for the quantitative assessment of ocular blood flow in white subjects. Our data affirms that the LSFG-derived variables FR and BOT may be useful biomarkers for age-related changes in ocular perfusion.
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DOI：
10.2147/opth.s22093
发表时间：
2011
期刊：
Clinical ophthalmology (Auckland, N.Z.)
影响因子：
--
作者：
Aizawa N;Yokoyama Y;Chiba N;Omodaka K;Yasuda M;Otomo T;Nakamura M;Fuse N;Nakazawa T
通讯作者：
Nakazawa T
影响因子：
1.6
作者：
Iwase T;Ra E;Yamamoto K;Kaneko H;Ito Y;Terasaki H
通讯作者：
Terasaki H
影响因子：
3.4
作者：
Baumann B;Potsaid B;Kraus MF;Liu JJ;Huang D;Hornegger J;Cable AE;Duker JS;Fujimoto JG
通讯作者：
Fujimoto JG
影响因子：
4.4
作者：
Cull, Grant;Burgoyne, Claude F.;Wang, Lin
通讯作者：
Wang, Lin
DOI：
10.1007/s00417-015-3247-0
发表时间：
2016-05-01
影响因子：
2.7
作者：
Kuroda, Yoshimasa;Uji, Akihito;Yoshimura, Nagahisa
通讯作者：
Yoshimura, Nagahisa