Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function.
Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function.
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三抗抗生素聚合物纳米纤维用于纳米药物递送：对双重物种生物膜和细胞功能的影响。
DOI：
10.1016/j.joen.2016.07.019
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发表时间：
2016-10
影响因子：
4.2
通讯作者：
Bottino MC
中科院分区：
文献类型：
--
作者：
Pankajakshan D;Albuquerque MT;Evans JD;Kamocka MM;Gregory RL;Bottino MC
根管消毒和建立有利于干细胞增殖/分化的根管内微环境在再生牙髓治疗中发挥着重要作用。本研究旨在（1）研究含三重抗生素的纳米纤维对双物种生物膜的抗菌功效，以及（2）评估牙髓干细胞（DPSC）在暴露于纳米纤维后在牙本质上粘附和增殖的能力。将在牙本质标本上建立的 7 天的双物种生物膜暴露于以下物质 3 天：盐水（对照）、不含抗生素的纳米纤维（对照）和含三重抗生素的纳米纤维或饱和三重抗生素糊剂 (TAP) 溶液（磷酸盐缓冲溶液中 50 mg/mL）。使用 LIVE/DEAD 测定（Molecular Probes, Inc, Eugene, OR）和共焦激光扫描显微镜评估细菌活力。对于细胞相容性研究，通过肌动蛋白-鬼笔环肽染色评估纳米纤维或 TAP（磷酸盐缓冲溶液中 1 g/mL）暴露后的牙本质标本的细胞粘附和扩散。在第 1、3 和 7 天评估 DPSC 增殖。进行统计，并将显着性设定为 5% 水平。共焦激光扫描显微镜显示，暴露于含抗生素的纳米纤维后，细菌显着死亡，与不含抗生素的纤维和对照（盐水）显着不同（P < .05）。与 TAP 对应物相比，DPSC 在用含抗生素纳米纤维处理的牙本质标本上表现出增强的粘附/铺展能力。不含抗生素的纳米纤维、含三重抗生素的纳米纤维和 TAP 处理的牙本质在第 1 天和第 3 天的 DPSC 增殖率相似。与 TAP 相比，第 7 天用含抗生素纳米纤维处理的牙本质的增殖更高（9 倍）。含有三重抗生素的聚合物纳米纤维会导致大量细菌死亡，但它们不会影响牙本质上的 DPSC 附着和增殖。
Root canal disinfection and the establishment of an intracanal microenvironment conducive to the proliferation/differentiation of stem cells play a significant role in regenerative endodontics. This study was designed to (1) investigate the antimicrobial efficacy of triple antibiotic–containing nanofibers against a dual-species biofilm and (2) evaluate the ability of dental pulp stem cells (DPSCs) to adhere to and proliferate on dentin upon nanofiber exposure. Seven-day-old dual-species biofilm established on dentin specimens was exposed for 3 days to the following: saline (control), antibiotic-free nanofibers (control), and triple antibiotic–containing nanofibers or a saturated triple antibiotic paste (TAP) solution (50 mg/mL in phosphate buffer solution). Bacterial viability was assessed using the LIVE/DEAD assay (Molecular Probes, Inc, Eugene, OR) and confocal laser scanning microscopy. For cyto-compatibility studies, dentin specimens after nanofiber or TAP (1 g/mL in phosphate buffer solution) exposure were evaluated for cell adhesion and spreading by actin-phalloidin staining. DPSC proliferation was assessed on days 1, 3, and 7. Statistics were performed, and significance was set at the 5% level. Confocal laser scanning microscopy showed significant bacterial death upon antibiotic-containing nanofiber exposure, differing significantly (P < .05) from antibiotic-free fibers and the control (saline). DPSCs showed enhanced adhesion/spreading on dentin specimens treated with antibiotic-containing nanofibers when compared with its TAP counterparts. The DPSC proliferation rate was similar on days 1 and 3 in antibiotic-free nanofibers, triple antibiotic–containing nanofibers, and TAP-treated dentin. Proliferation was higher (9-fold) on dentin treated with antibiotic-containing nanofibers on day 7 compared with TAP. Triple antibiotic–containing polymer nanofibers led to significant bacterial death, whereas they did not affect DPSC attachment and proliferation on dentin.
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影响因子：
3
作者：
Roberts-Clark, DJ;Smith, AJ
通讯作者：
Smith, AJ
影响因子：
4.2
作者：
Ruparel, Nikita B.;Teixeira, Fabricio B.;Diogenes, Anibal
通讯作者：
Diogenes, Anibal
影响因子：
1.9
作者：
Waeiss, Robert A.;Negrini, Thais C.;Bottino, Marco C.
通讯作者：
Bottino, Marco C.
影响因子：
4.2
作者：
Distel, JW;Hatton, JF;Gillespie, MJ
通讯作者：
Gillespie, MJ
抗菌TAP-MIMIC电纺聚合物支架：对牙龈疟原虫感染的牙本质生物膜的影响。
影响因子：
3.4
作者：
Albuquerque MT;Evans JD;Gregory RL;Valera MC;Bottino MC
通讯作者：
Bottino MC