EVALUATION OF THE ANTITUMOR ACTIVITY BY CdTe QDs WITH VERBASCOSIDE
EVALUATION OF THE ANTITUMOR ACTIVITY BY CdTe QDs WITH VERBASCOSIDE
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CdTe量子点与毛蕊花苷的抗肿瘤活性评价
DOI：
10.1142/s1793292013500318
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发表时间：
2013-06
期刊：
影响因子：
1.2
通讯作者：
Zhang, Gen
中科院分区：
文献类型：
--
作者：
Yue, Hui-Lan;Li, Ping;Zeng, Xin（曾新;共同通讯作者）;Zhang, Gen
碲化镉量子点（QD）因其在药物输送方面的潜力而在生物医学研究中受到了极大的关注。在本研究中，化疗剂毛蕊花苷（VB）通过VB的-OH基团和CdTe QDs的-COOH基团之间的共价键合成功地固定在CdTe QDs上。可以固定的 VB 量取决于 CdTe QD 表面上的 -COOH 基团（琥珀酸）的浓度。凋亡染色、DNA片段化和流式细胞术分析进一步表明，与单独CdTe QDs或VB处理相比，CdTe QDs与VB（VB-QDs）联合处理HepG2/ADM细胞后细胞凋亡率增加。我们观察到 VB-QD 处理可以明显激活 HepG2/ADM 细胞中凋亡相关的 Caspase 3 表达。此外，我们的体内研究表明，VB-QDs的治疗有效抑制人肝癌HepG2/ADM裸鼠肿瘤的生长。在研究的动物中，细胞凋亡率的增加与肿瘤生长的抑制作用的增强密切相关。因此，VB-QD 可以作为某些癌症治疗方法的可能替代方案。总之，研究表明，基于细胞凋亡的分析，VB-QDs 纳米颗粒的存在可以治疗癌细胞。
Cadmium telluride quantum dots (QDs) have received significant attention in biomedical research because of their potential in drug delivery. In this study, the chemotherapeutic agent Verbascoside (VB) was immobilized successfully onto CdTe QDs by covalent bonding between the -OH group of VB and the -COOH group of CdTe QDs. The amount of VB that could be immobilized depended on the concentration of -COOH groups (succinic acid) on the surface of the CdTe QDs. Apoptotic staining, DNA fragmentation and flow cytometry analysis further demonstrated that compared with CdTe QDs or VB treatment alone, the apoptosis rate increased after the treatment of CdTe QDs together with VB (VB-QDs) in HepG2/ADM cells. We observed that VB-QDs treatment could clearly activate apoptosis-related Caspase 3 expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of VB-QDs effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, VB-QDs may serve as a possible alternative for therapeutic approaches for some cancer treatments. In summary, studies have shown that cancer cells are treated by the presence of VB-QDs nanoparticles based on analysis of cell apoptosis.
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影响因子：
7.2
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
14
作者：
Shao, Dan;Zeng, Qinghui;Zhang, Hong
通讯作者：
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影响因子：
1.2
作者：
Gen Zhang;Hui Jiang;Xuemei Wang
通讯作者：
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