Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering.
Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering.
复制标题
DOI：
10.3390/ma14174773
复制
发表时间：
2021-08-24
期刊：
影响因子：
--
通讯作者：
Sefat F
中科院分区：
文献类型：
--
作者：
Bazgir M;Zhang W;Zhang X;Elies J;Saeinasab M;Coates P;Youseffi M;Sefat F
本研究旨在评估聚乳酸-羟基乙酸共聚物（PLGA）和聚己内酯（PCL）纳米纤维支架的特性和降解对结构性能的影响。通过静电纺丝制备六个支架，三个与PCL 15%（w/v）和三个与PLGA 10%（w/v），与静电纺丝处理时间为30，60和90分钟。这两种类型的支架显示更强大的机械性能，增加纺丝时间。具有90分钟电纺膜的两种支架的拉伸强度在其强度上没有显示出显著差异，因为PCL和PLGA支架分别测量为1.492 MPa ± 0.378 SD和1.764 MPa ± 0.7982 SD。所有膜在润湿性测试下显示为疏水性的。通过将所有支架在室温下浸入磷酸盐缓冲盐水（PBS）溶液中12周和在37 °C下浸入4周来进行降解行为研究。通过每周从PBS溶液中取出每个样品来监测降解效果，并在扫描电子显微镜（SEM）下研究结构变化。PCL和PLGA支架显示出优异的纤维结构，具有足够的降解，并且随着时间的推移测量的纤维直径显示尺寸略有增加。因此，作为纤维吸水和逐渐降解的一个例子，支架的重量损失百分比每周都在增加，进一步支持多孔膜的可降解性。所有支架的孔径和孔隙率百分比在降解期间大幅下降。从该实验得出的结论是，PCL和PLGA在组织工程和再生医学应用中具有很大的前景。
The current study aimed to evaluate the characteristics and the effects of degradation on the structural properties of Poly(lactic-co-glycolic acid) (PLGA)- and polycaprolactone (PCL)-based nanofibrous scaffolds. Six scaffolds were prepared by electrospinning, three with PCL 15% (w/v) and three with PLGA 10% (w/v), with electrospinning processing times of 30, 60 and 90 min. Both types of scaffolds displayed more robust mechanical properties with increased spinning times. The tensile strength of both scaffolds with 90-min electrospun membranes did not show a significant difference in their strengths, as the PCL and PLGA scaffolds measured at 1.492 MPa ± 0.378 SD and 1.764 MPa ± 0.7982 SD, respectively. All membranes were shown to be hydrophobic under a wettability test. A degradation behaviour study was performed by immersing all scaffolds in phosphate-buffered saline (PBS) solution at room temperature for 12 weeks and for 4 weeks at 37 °C. The effects of degradation were monitored by taking each sample out of the PBS solution every week, and the structural changes were investigated under a scanning electron microscope (SEM). The PCL and PLGA scaffolds showed excellent fibre structure with adequate degradation, and the fibre diameter, measured over time, showed slight increase in size. Therefore, as an example of fibre water intake and progressive degradation, the scaffold’s percentage weight loss increased each week, further supporting the porous membrane’s degradability. The pore size and the porosity percentage of all scaffolds decreased substantially over the degradation period. The conclusion drawn from this experiment is that PCL and PLGA hold great promise for tissue engineering and regenerative medicine applications.
登录
查看更多内容
DOI：
10.1002/jbm.b.30683
发表时间：
2007-05-01
影响因子：
3.4
作者：
Diego, Raul Brigido;Estelles, Jorge Mas;Sanchez, Manuel Salmeron
通讯作者：
Sanchez, Manuel Salmeron
影响因子：
--
作者：
He, W;Yong, T;Ramakrishna, S
通讯作者：
Ramakrishna, S
影响因子：
19
作者：
Agarwal, Seema;Greiner, Andreas;Wendorff, Joachin H.
通讯作者：
Wendorff, Joachin H.
影响因子：
6.2
作者：
Alves da Silva, M. L.;Martins, A.;Neves, N. M.
通讯作者：
Neves, N. M.
影响因子：
0.1
作者：
Bye, Frazer J.;Bullock, Anthony J.;MacNeil, Sheila
通讯作者：
MacNeil, Sheila