Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia.
Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia.
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DOI：
10.1371/journal.pone.0203064
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Vukojević J
中科院分区：
文献类型：
--
作者：
Knežević A;Stajić M;Sofrenić I;Stanojković T;Milovanović I;Tešević V;Vukojević J
在过去的几十年里，蘑菇作为功能性食品和生物活性化合物的来源越来越受到关注。几个栓菌属物种已被用于东亚文化的传统医学几个世纪，但只有T。对变色龙的研究已经很充分，而对其他物种的药用特性的实质性数据较少。变色栓菌（Trametes versicolor）、T. hirsuta和T. gibbosa是测试生物活性的物种。提取物的抗真菌潜力进行了评估，为选定的念珠菌和曲霉属的临床菌株。ABTS和FRAP测定被用来评估所研究的提取物的抗氧化能力。测定了对人宫颈癌、肺腺癌和结肠癌细胞系的细胞毒活性。通过测定乙酰胆碱酯酶和酪氨酸酶活性的速率来评估抗神经变性活性。通过1H NMR光谱分析研究栓菌属物种的菌丝体和担子果提取物中代谢产物的存在。研究的提取物显示出较低的抗真菌潜力与酮康唑相比。担子果提取物对ABTS+的清除和Fe ~（2+）的还原效果优于菌丝体提取物，但不如L-抗坏血酸。结果表明，菌丝体提取物对3种癌细胞株的细胞毒作用均强于担子果提取物，且宫颈腺癌细胞对菌丝体提取物和市售细胞抑制剂最敏感。T.云芝菌丝体提取物对乙酰胆碱酯酶活性的抑制作用最强，但比加兰他敏弱一倍;赤霉菌丝体提取物对酪氨酸酶活性的抑制效果显著优于曲酸，抑制率为40.9%。化学分析表明，三萜，糖和多酚在应用的测定中具有很强的协同作用。结果表明，测试栓菌物种具有显着的药用潜力，这可能是由于抗氧化和细胞毒活性。此外，担子果和菌丝体提取物对乙酰胆碱酯酶和酪氨酸酶的活性均有较强的抑制作用。
In a last few decades mushrooms are increasingly attracting attention as functional food and sources of biologically active compounds. Several Trametes species have been used for centuries in traditional medicine of East Asia cultures, but only T. versicolor was studied sufficiently while there are less substantial data about medicinal properties of other species. Trametes versicolor, T. hirsuta and T. gibbosa were the species tested for biological activities. Antifungal potentials of extracts were assessed for clinical strains of selected Candida and Aspergillus species. ABTS and FRAP assays were used to evaluate antioxidant capacities of studied extracts. Cytotoxic activity was determined against human cervix and lung adenocarcinoma and colon carcinoma cell lines. Antineurodegenerative activity was assessed by determining the rate of acetylcholinesterase and tyrosinase activity. The presence of metabolites in extracts of mycelia and basidiocarps of studied Trametes species was analyzed by 1H NMR spectroscopy. Studied extracts showed low antifungal potential in comparison with ketoconazole. Basidiocarp extracts were more effective ABTS+ scavengers and Fe2+ reducers than mycelium ones but less effective in comparison with L-ascorbic acid. Results showed that mycelium extracts had stronger cytotoxic effects against three cancer cell lines than basidiocarp ones, and that cervix adenocarcinoma cells were the most sensitive to the extracts and commercial cytostatics. T. versicolor mycelium extract was the most effective inhibitor of acetylcholinesterase activity but double weaker than galantamine, and T. gibbosa mycelium extract was significantly better inhibitor of tyrosinase activity than kojic acid for 40.9%. Chemical analysis indicated strong synergistic action of triterpenes, sugars and polyphenols in applied assays. The results suggest that tested Trametes species have significant medicinal potentials which could be attributed to antioxidative and cytotoxic activity. Additionally both, basidiocarps and mycelia extracts can strongly inhibit activity of acetylcholinesterase and tyrosinase.
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