Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy.
Controlling Adult Stem Cell Behavior Using Nanodiamond-Reinforced Hydrogel: Implication in Bone Regeneration Therapy.
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DOI：
10.1038/s41598-017-06028-y
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发表时间：
2017-07-26
影响因子：
4.6
通讯作者：
Paul A
中科院分区：
文献类型：
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作者：
Pacelli S;Maloney R;Chakravarti AR;Whitlow J;Basu S;Modaresi S;Gehrke S;Paul A
纳米金刚石（NDs）由于其低细胞毒性和易于表面功能化的特点，作为药物递送的纳米载体受到了广泛的关注。鉴于这些特点，NDs最近被研究用于组织工程纳米复合水凝胶的制备。在这里，我们报道了一种水凝胶的合成，使用光交联明胶甲基丙烯酰胺（GelMA）和nd作为药物传递和干细胞引导骨再生的三维支架。研究了不同浓度nd对凝胶网络物理力学性能的影响。nd的加入增加了网络刚度，这反过来又增加了人类脂肪干细胞（hASCs）产生的牵引力。我们还测试了NDs吸附和调节模型药物地塞米松（Dex）释放的能力，以促进hASCs的成骨分化。ND-Dex复合物可调节hASCs的基因表达、细胞面积和局灶黏附数。此外，ND-Dex复合物在GelMA水凝胶中的整合使得Dex随时间的保留率更高，从而显着增加了包封hASCs的碱性磷酸酶活性和钙沉积。这些结果表明，传统的GelMA水凝胶可以与共轭ndds偶联，为骨组织工程开发一个新的平台。
Nanodiamonds (NDs) have attracted considerable attention as drug delivery nanocarriers due to their low cytotoxicity and facile surface functionalization. Given these features, NDs have been recently investigated for the fabrication of nanocomposite hydrogels for tissue engineering. Here we report the synthesis of a hydrogel using photocrosslinkable gelatin methacrylamide (GelMA) and NDs as a three-dimensional scaffold for drug delivery and stem cell-guided bone regeneration. We investigated the effect of different concentration of NDs on the physical and mechanical properties of the GelMA hydrogel network. The inclusion of NDs increased the network stiffness, which in turn augmented the traction forces generated by human adipose stem cells (hASCs). We also tested the ability of NDs to adsorb and modulate the release of a model drug dexamethasone (Dex) to promote the osteogenic differentiation of hASCs. The ND-Dex complexes modulated gene expression, cell area, and focal adhesion number in hASCs. Moreover, the integration of the ND-Dex complex within GelMA hydrogels allowed a higher retention of Dex over time, resulting in significantly increased alkaline phosphatase activity and calcium deposition of encapsulated hASCs. These results suggest that conventional GelMA hydrogels can be coupled with conjugated NDs to develop a novel platform for bone tissue engineering.
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影响因子：
6.7
作者：
Moore L;Grobárová V;Shen H;Man HB;Míčová J;Ledvina M;Štursa J;Nesladek M;Fišerová A;Ho D
通讯作者：
Ho D
DOI：
10.1073/pnas.0801866105
发表时间：
2008-07-15
影响因子：
11.1
作者：
Du, Yanan;Lo, Edward;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
29.4
作者：
Gaharwar, Akhilesh K.;Mihaila, Silvia M.;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
13.3
作者：
Alhaddad, Anna;Adam, Marie-Pierre;Bertrand, Jean-Remi
通讯作者：
Bertrand, Jean-Remi
影响因子：
38.3
作者：
通讯作者：
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