Acoustic droplet-hydrogel composites for spatial and temporal control of growth factor delivery and scaffold stiffness.
Acoustic droplet-hydrogel composites for spatial and temporal control of growth factor delivery and scaffold stiffness.
复制标题
DOI：
10.1016/j.actbio.2013.03.027
复制
发表时间：
2013-07
影响因子：
9.7
通讯作者：
Franceschi, Renny T.
中科院分区：
文献类型：
--
作者：
Fabiilli, Mario L.;Wilson, Christopher G.;Padilla, Frederic;Martin-Saavedra, Francisco M.;Fowlkes, J. Brian;Franceschi, Renny T.
伤口愈合受生长因子信号的时间和空间限制模式的调节，但很少有能够“按需”释放这些模式所必需的递送载体。最近，我们描述了一种全氟碳双乳液，该乳液在暴露于超声波时通过称为声滴汽化（ADV）的过程选择性地释放蛋白质负载。在这项研究中，我们描述了一种由纤维蛋白水凝胶掺杂生长因子负载的双乳液组成的递送系统，用于组织再生。ADV后，复合材料中免疫反应性碱性成纤维细胞生长因子（bFGF）的释放量增加了5倍，并且通过延迟暴露于超声而延迟释放。与假对照相比，超声处理材料的释放显著增加了内皮细胞的增殖，表明释放的bFGF具有生物活性。ADV还引发了纤维蛋白的超微结构和力学性能的变化，因为在超声处理的复合材料中，纤维蛋白的气泡形成和巩固伴随着高达22倍的剪切刚度增加。ADV并未降低悬浮在复合支架中的细胞活力。这些结果表明，通过按需控制生长因子释放和/或支架结构，声滴-水凝胶复合材料在促进伤口愈合方面具有广泛的应用。
Wound healing is regulated by temporally and spatially restricted patterns of growth factor signaling, but there are few delivery vehicles capable of the “on-demand” release necessary for recapitulating these patterns. Recently we described a perfluorocarbon double emulsion that selectively releases a protein payload upon exposure to ultrasound through a process known as acoustic droplet vaporization (ADV). In this study, we describe a delivery system composed of fibrin hydrogels doped with growth factor-loaded double emulsion for applications in tissue regeneration. Release of immunoreactive basic fibroblast growth factor (bFGF) from the composites increased up to 5-fold following ADV and delayed release was achieved by delaying exposure to ultrasound. Releasates of ultrasound-treated materials significantly increased the proliferation of endothelial cells compared to sham controls, indicating that the released bFGF was bioactive. ADV also triggered changes in the ultrastructure and mechanical properties of the fibrin as bubble formation and consolidation of the fibrin in ultrasound-treated composites were accompanied by up to a 22-fold increase in shear stiffness. ADV did not reduce the viability of cells suspended in composite scaffolds. These results demonstrate that an acoustic droplet–hydrogel composite could have broad utility in promoting wound healing through on-demand control of growth factor release and/or scaffold architecture.
登录
查看更多内容
影响因子：
3.7
作者：
Fabiilli, Mario L.;Lee, James A.;Kripfgans, Oliver D.;Carson, Paul L.;Fowlkes, J. Brian
通讯作者：
Fowlkes, J. Brian
DOI：
10.1016/j.colsurfa.2004.06.003
发表时间：
2004-09-06
影响因子：
5.2
作者：
Courrier, HA;Vandamme, TF;Krafft, MP
通讯作者：
Krafft, MP
影响因子：
4.9
作者：
Frimpong, Reynolds A.;Fraser, Stew;Hilt, J. Zach
通讯作者：
Hilt, J. Zach
影响因子：
2.9
作者：
Garvin, Kelley A.;Hocking, Denise C.;Dalecki, Diane
通讯作者：
Dalecki, Diane
DOI：
10.1109/tuffc.2009.1132
发表时间：
2009-05
期刊：
IEEE transactions on ultrasonics, ferroelectrics, and frequency control
影响因子：
--
作者：
Fabiilli ML;Haworth KJ;Fakhri NH;Kripfgans OD;Carson PL;Fowlkes JB
通讯作者：
Fowlkes JB