Identification of a DNA segment that is necessary and sufficient for alpha-specific gene control in Saccharomyces cerevisiae: implications for regulation of alpha-specific and a-specific genes.
Identification of a DNA segment that is necessary and sufficient for alpha-specific gene control in Saccharomyces cerevisiae: implications for regulation of alpha-specific and a-specific genes.
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鉴定酿酒酵母中 α 特异性基因控制所必需和充分的 DNA 片段：对 α 特异性和 α 特异性基因调控的影响。
DOI：
10.1128/mcb.8.1.309-320.1988
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发表时间：
1988
影响因子：
5.3
通讯作者：
SpragueJr,GF
中科院分区：
文献类型：
--
作者：
Jarvis,EE;Hagen,DC;SpragueJr,GF
STE 3 mRNA仅存在于酿酒酵母α细胞中，而不存在于α或α/α细胞中，并且当用α因子交配信息素处理细胞时，转录水平增加约5倍。STE 3 5′端非编码区的缺失定义了一个43个碱基对（bp）的上游激活序列（UAS），当取代天然CYC 1 UAS时，该序列可赋予CYC 1-lacZ融合体两种调控模式。UAS活性需要MATα的α1产物，已知该产物是α特异性基因转录所需的。仅去除14 bp的STE 3 UAS的染色体缺失使STE 3转录水平降低50至100倍，表明UAS对于表达是必需的。STE 3 UAS与其他已知的α特异性基因MFα1和MFα2的5′端非编码序列具有26 bp的同源性。我们认为同源性有两个组成部分-一个近回文的16 bp的“P盒”和一个相邻的10 bp的“Q盒”。合成的STE 3 P盒作为UAS是无活性的;完美的回文P盒在所有三种细胞类型中都是活性的。我们认为P盒是转录激活因子的结合位点，但是α1通过Q盒起作用是该激活因子与α特异性基因的不完全P盒结合所必需的。在α-特异性基因的上游也发现了P盒的版本，位于MATα编码的阻遏物α2的结合位点内。因此，MATα的产物可以通过控制相同的转录激活因子进入其结合位点P盒而使基因表达α或α特异性。
STE3 mRNA is present only in Saccharomyces cerevisiae α cells, not in a or a/α cells, and the transcript level increases about fivefold when cells are treated with a-factor mating pheromone. Deletions in the 5′ noncoding region of STE3 defined a 43-base-pair (bp) upstream activation sequence (UAS) that can impart both modes of regulation to a CYC1-lacZ fusion when substituted for the native CYC1 UAS. UAS activity required the α1 product of MATα, which is known to be required for transcription of α-specific genes. A chromosomal deletion that removed only 14 bp of the STE3 UAS reduced STE3 transcript levels 50- to 100-fold, indicating that the UAS is essential for expression. The STE3 UAS shares a 26-bp homology with the 5′ noncoding sequences of the only other known α-specific genes, MFα1 and MFα2. We view the homology as having two components—a nearly palindromic 16-bp “P box” and an adjacent 10-bp “Q box.” A synthetic STE3 P box was inactive as a UAS; a perfect palindrome P box was active in all three cell types. We propose that the P box is the binding site for a transcription activator, but that α1 acting via the Q box is required for this activator to bind to the imperfect P boxes of α-specific genes. Versions of the P box are also found upstream of a-specific genes, within the binding sites of the repressor α2 encoded by MATα. Thus, the products of MATα may render gene expression α or a-specific by controlling access of the same transcription activator to its binding site, the P box.
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影响因子：
3.5
作者：
BOTSTEIN, D;FALCO, SC;DAVIS, RW
通讯作者：
DAVIS, RW
酿酒酵母交配型特异性基因 STE2 的多重调控
影响因子：
5.3
作者：
A. Hartig;J. Holly;G. Saari;V. Mackay
通讯作者：
V. Mackay
酿酒酵母中的细胞相互作用和细胞类型的调节。
影响因子：
10.5
作者：
G. Sprague;L. Blair;J. Thorner
通讯作者：
J. Thorner
影响因子：
5.6
作者：
MCNEIL, JB;SMITH, M
通讯作者：
SMITH, M
影响因子：
64.5
作者：
JENNESS, DD;BURKHOLDER, AC;HARTWELL, LH
通讯作者：
HARTWELL, LH